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Identification of a novel insertion sequence in vanB2-containing Enterococcus faecium.

DC Field Value Language
dc.contributor.authorLee, WG-
dc.contributor.authorKim, W-
dc.date.accessioned2011-07-26T05:49:14Z-
dc.date.available2011-07-26T05:49:14Z-
dc.date.issued2003-
dc.identifier.issn0266-8254-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/3597-
dc.description.abstractAIMS: The characterization of a novel insertion sequence (IS) in vanB2-containing Enterococcus faecium was conducted.



METHODS AND RESULTS: Direct PCR amplification of ORFC region of Tn5382 from DNA extracted from vanB2-containing E. faecium, and sequence analysis were performed. A novel IS was identified. It is 1418 bp in length and contains one putative open reading frame that is similar to transposase. There exists inverted terminal repeats of 12 bp, but direct repeats are not present. According to high similarity to putative transposases of IS3 members, such as, IS150, IS861, IS1077 and IS911, we designated it ISEnfa3.



SIGNIFICANCE AND IMPACT OF STUDY: Since ISEnfa3 was detected in all vanB2-containing strains examined so far, it could be used as a tool for epidemiological study.
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dc.language.isoen-
dc.subject.MESHAmino Acid Sequence-
dc.subject.MESHAnimals-
dc.subject.MESHBacterial Proteins/genetics*-
dc.subject.MESHBase Sequence-
dc.subject.MESHChromosome Mapping-
dc.subject.MESHDNA Primers/genetics-
dc.subject.MESHDNA Transposable Elements/genetics*-
dc.subject.MESHDNA, Bacterial/genetics-
dc.subject.MESHEnterococcus/drug effects-
dc.subject.MESHEnterococcus/genetics-
dc.subject.MESHEnterococcus/isolation & purification-
dc.subject.MESHEnterococcus faecalis/drug effects-
dc.subject.MESHEnterococcus faecalis/genetics-
dc.subject.MESHEnterococcus faecalis/isolation & purification-
dc.subject.MESHEnterococcus faecium/drug effects-
dc.subject.MESHEnterococcus faecium/genetics*-
dc.subject.MESHEnterococcus faecium/isolation & purification-
dc.subject.MESHGenes, Bacterial*-
dc.subject.MESHGenetic Linkage-
dc.subject.MESHHumans-
dc.subject.MESHMolecular Sequence Data-
dc.subject.MESHMultigene Family-
dc.subject.MESHMutagenesis, Insertional-
dc.subject.MESHSequence Homology, Amino Acid-
dc.titleIdentification of a novel insertion sequence in vanB2-containing Enterococcus faecium.-
dc.typeArticle-
dc.identifier.pmid12581381-
dc.identifier.urlhttp://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0266-8254&date=2003&volume=36&issue=3&spage=186-
dc.contributor.affiliatedAuthor이, 위교-
dc.type.localJournal Papers-
dc.citation.titleLetters in applied microbiology-
dc.citation.volume36-
dc.citation.number3-
dc.citation.date2003-
dc.citation.startPage186-
dc.citation.endPage190-
dc.identifier.bibliographicCitationLetters in applied microbiology, 36(3):186-190, 2003-
dc.identifier.eissn1472-765X-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Laboratory Medicine
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