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Separation of triacylglycerols and free fatty acids in microalgal lipids by solid-phase extraction for separate fatty acid profiling analysis by gas chromatography.

Authors
Paik, MJ; Kim, H; Lee, J; Brand, J; Kim, KR
Citation
Journal of chromatography. A, 1216(31):5917-5923, 2009
Journal Title
Journal of chromatography. A
ISSN
0021-96731873-3778
Abstract
Microalgal lipids were separated into two fractions, triacylglycerols (TAGs) and free fatty acids (FFAs), by solid-phase extraction employing sodium carbonate as the sorbent and dichloromethane (20% by volume) in n-hexane as the extracting solvent. The TAG fraction was then saponified, followed by acidification, extraction and tert-butyldimethylsilyl esterification. The FFA fraction was directly acidified, extracted and derivatized. From the lipid extracts of eight microalgal species examined, a total of 13 fatty acids were detected in the TAG fractions and nine were found in the FFA fractions, with at much higher total TAG content in all microalgae. Oleic acid was the most prominent fatty acid in three species, alpha-linolenic acid was more abundant in two others, and palmitic acid was present in highest concentration in the remaining three species.
MeSH terms
Algae/chemistry*Carbonates/chemistryChromatography, Gas/methods*Fatty Acids, Nonesterified/analysis*Mass Spectrometry/methodsOrganosilicon CompoundsRegression AnalysisReproducibility of ResultsSolid Phase Extraction/methods*Triglycerides/analysis*
DOI
10.1016/j.chroma.2009.06.051
PMID
19570540
Appears in Collections:
Journal Papers > Research Organization > Institute for Neuroregeneration & Stem Cell Research
AJOU Authors
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