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KR-31466, a benzopyranylindol analog, attenuates hypoxic injury through mitochondrial K(ATP) channel and protein kinase C activation in heart-derived H9c2 cells.

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dc.contributor.authorJung, YS-
dc.contributor.authorJung, YS-
dc.contributor.authorKim, MY-
dc.contributor.authorKim, MH-
dc.contributor.authorLee, S-
dc.contributor.authorYi, KY-
dc.contributor.authorYoo, SE-
dc.contributor.authorLee, SH-
dc.contributor.authorBaik, EJ-
dc.contributor.authorMoon, CH-
dc.contributor.authorCho, JP-
dc.date.accessioned2011-07-26T06:36:16Z-
dc.date.available2011-07-26T06:36:16Z-
dc.date.issued2003-
dc.identifier.issn1347-8613-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/3600-
dc.description.abstractIn the present study, we investigated whether a novel benzopyranylindol analogue, KR-31466 (KR466) (1-[(2S,3R,4S)-3,4-dihydro-2-dimethoxymethyl-3-hydroxy-2-methyl-6-nitro-2H-1-benzopyran-4-yl]-1H-indole-2-carboxylic acid ethyl ester) can attenuate hypoxic injury in heart-derived H9c2 cells and, if so, whether the protective effect of KR466 is mediated through mitochondrial ATP-sensitive potassium (mtK(ATP)) opening. The treatment of H9c2 cells with KR466 (3 - 30 microM) significantly reduced hypoxia-induced cell death in a concentration-dependent manner, as shown by lactate dehydrogenase release and propidium iodide-uptake. In addition, KR466 (10 microM) significantly reduced the increase in hypoxia-induced TUNEL-positive cells, suggesting its anti-apoptotic potential in H9c2 cells. The protective effects of KR466 were abolished by 5-hydroxydecanoate, a specific blocker of the mtK(ATP) channel, suggesting the involvement of the mtK(ATP) channel in the protective effect of KR466. A specific inhibitor of protein kinase C (PKC), chelerythrine (3 microM), significantly attenuated the protective effect of KR466 against hypoxia-induced cardiac cell death. In conclusion, our results suggest that KR466 can protect H9c2 cells from hypoxia-induced death through mtK(ATP) channel opening and PKC activation.-
dc.language.isoen-
dc.subject.MESHAnimals-
dc.subject.MESHBenzopyrans-
dc.subject.MESHCell Hypoxia-
dc.subject.MESHCell Line-
dc.subject.MESHEnzyme Activation-
dc.subject.MESHIn Situ Nick-End Labeling-
dc.subject.MESHIndicators and Reagents-
dc.subject.MESHIndoles-
dc.subject.MESHL-Lactate Dehydrogenase-
dc.subject.MESHMembrane Proteins-
dc.subject.MESHMyocardium-
dc.subject.MESHPotassium Channels-
dc.subject.MESHPropidium-
dc.subject.MESHProtective Agents-
dc.subject.MESHProtein Kinase C-
dc.subject.MESHRats-
dc.titleKR-31466, a benzopyranylindol analog, attenuates hypoxic injury through mitochondrial K(ATP) channel and protein kinase C activation in heart-derived H9c2 cells.-
dc.typeArticle-
dc.identifier.pmid12832850-
dc.identifier.urlhttp://joi.jlc.jst.go.jp/JST.JSTAGE/jphs/92.13?from=PubMed-
dc.contributor.affiliatedAuthor정, 이숙-
dc.contributor.affiliatedAuthor정, 윤석-
dc.contributor.affiliatedAuthor이, 수환-
dc.contributor.affiliatedAuthor백, 은주-
dc.contributor.affiliatedAuthor문, 창현-
dc.contributor.affiliatedAuthor조, 준필-
dc.type.localJournal Papers-
dc.citation.titleJournal of pharmacological sciences-
dc.citation.volume92-
dc.citation.number1-
dc.citation.date2003-
dc.citation.startPage13-
dc.citation.endPage18-
dc.identifier.bibliographicCitationJournal of pharmacological sciences, 92(1). : 13-18, 2003-
dc.identifier.eissn1347-8648-
dc.relation.journalidJ013478613-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Physiology
Journal Papers > School of Medicine / Graduate School of Medicine > Emergency Medicine
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