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Differential expression of cell surface proteins in human bone marrow mesenchymal stem cells cultured with or without basic fibroblast growth factor containing medium.

Authors
Lee, SK; Kim, Y; Kim, SS; Lee, JH; Cho, K; Lee, SS; Lee, ZW; Kwon, KH; Kim, YH; Suh-Kim, H; Yoo, JS; Park, YM
Citation
Proteomics, 9(18):4389-4405, 2009
Journal Title
Proteomics
ISSN
1615-98531615-9861
Abstract
Mesenchymal stem cells (MSCs) are multipotent cells, which have the capability to differentiate into various mesenchymal tissues such as bone, cartilage, fat, tendon, muscle, and marrow stroma. However, they lose the capability of multi-lineage differentiation after several passages. It is known that basic fibroblast growth factor (bFGF) increases growth rate, differentiation potential, and morphological changes of MSCs in vitro. In this report, we have used 2-DE coupled to MS to identify differentially expressed proteins at the cell membrane level in MSCs growing in bFGF containing medium. The cell surface proteins isolated by the biotin-avidin affinity column were separated by 2-DE in triplicate experiments. A total of 15 differentially expressed proteins were identified by quadrupole-time of flight tandem MS. Nine of the proteins were upregulated and six proteins were downregulated in the MSCs cultured with bFGF containing medium. The expression level of three actin-related proteins, F-actin-capping protein subunit alpha-1, actin-related protein 2/3 complex subunit 2, and myosin regulatory light chain 2, was confirmed by Western blot analysis. The results indicate that the expression levels of F-actin-capping protein subunit alpha-1, actin-related protein 2/3 complex subunit 2, and myosin regulatory light chain 2 are important in bFGF-induced morphological change of MSCs.
MeSH terms
Actins/metabolismBlotting, WesternBone Marrow Cells/cytology*Bone Marrow Cells/drug effectsBone Marrow Cells/metabolismCells, CulturedCulture MediaElectrophoresis, Gel, Two-DimensionalFibroblast Growth Factor 2/pharmacology*HumansImmunohistochemistryMembrane Proteins/biosynthesis*Mesenchymal Stem Cells/drug effectsMesenchymal Stem Cells/metabolism*Proteins/metabolismProteomics/methods*Reproducibility of ResultsTandem Mass Spectrometry
DOI
10.1002/pmic.200900165
PMID
19655310
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Journal Papers > School of Medicine / Graduate School of Medicine > Anatomy
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