17 270

Cited 0 times in

An endogenous proteinacious inhibitor in porcine liver for S-adenosyl-L-methionine dependent methylation reactions: identification as oligosaccharide-linked acyl carrier protein.

Seo, DW; Moon, HI; Han, JW; Hong, SY; Lee, HY; Kim, S; Paik, WK; Lee, HW
The international journal of biochemistry & cell biology, 32(4):455-464, 2000
Journal Title
The international journal of biochemistry & cell biology
A proteinacious inhibitor of S-adenosyl-L-methionine (AdoMet)-dependent transmethylation reactions was purified to homogeneity from porcine liver by size exclusion chromatography and FPLC. The molecular weight of the inhibitor was 12,222 Da. A 7400 Da polypeptide fragment of the purified inhibitor was sequenced by matrix-associated laser desorption ionization; time-of-flight MS, and was found to be identical with the known sequence of spinach acyl carrier protein (ACP). Although the remainder of the molecule was not clearly defined, 1H and H-H correlation of spectroscopy (COSY) NMR analysis revealed the presence of an oligosaccharide with alpha-glycosidic linkage. The purified oligosaccharide-linked ACP inhibited several AdoMet-dependent transmethylation reactions such as protein methylase I and II. S-farnesylcysteine O-methyltransferase, DNA methyltransferase and phospholipid methyltransferase. Protein methylase II was inhibited with a Ki value of 2.4 x 10(-3) M in a mixed inhibition pattern, whereas a well-known competitive product inhibitor S-adenosyl-L-homocysteine (AdoHcy) had Ki value of 6.3 x 10(-6) M. Commercially available active ACP fragments (65-74) and ACP from Escherichia coli had less inhibitory activity toward S-farnesylcysteine O-methyltransferase than the purified inhibitor. The biological significance of this oligosaccharide-linked ACP which has two seemingly unrelated functions (inhibitor for transmethylation and fatty acid biosynthesis) remains to be elucidated.
MeSH terms
Acyl Carrier Protein/*chemistry/isolation & purificationAnimalsEnzyme Inhibitors/chemistry/isolation & purificationKineticsLiver/*chemistryMagnetic Resonance SpectroscopyMethylationMethyltransferases/antagonists & inhibitors/chemistryMolecular WeightOligosaccharides/*chemistryPeptide Fragments/chemistryProtein Methyltransferases/chemistryRatsS-Adenosylmethionine/*chemistrySpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationSwineTransferases (Other Substituted Phosphate Groups)/chemistry
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Biochemistry & Molecular Biology
AJOU Authors
백, 운기
Full Text Link
RIS (EndNote)
XLS (Excel)


해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.