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Association of human kinesin superfamily protein member 4 with BRCA2-associated factor 35.

DC Field Value Language
dc.contributor.authorLee, YM-
dc.contributor.authorKim, W-
dc.date.accessioned2011-07-27T01:28:01Z-
dc.date.available2011-07-27T01:28:01Z-
dc.date.issued2003-
dc.identifier.issn0264-6021-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/3618-
dc.description.abstractA large portion of human kinesin superfamily protein member 4 (KIF4) is associated with the nuclear matrix during the interphase, while a small portion is found in the cytoplasm. During mitosis, it is associated with chromosomes throughout the entire process. In the present study, we identified a protein that interacts with KIF4 using a yeast two-hybrid system, co-immunoprecipitation and co-fractionation. This protein is BRCA2-associated factor 35 (BRAF35) containing a non-specific DNA binding high-mobility-group domain and a kinesin-like coiled-coil domain. It appeared that the interaction between the two proteins occurs through their respective alpha-helical coiled-coil domains. The co-fractionation experiment revealed that KIF4 and BRAF35 were present in a complex of approx. 540 kDa. The composition and biological significance of this complex should be studied further.-
dc.language.isoen-
dc.subject.MESHAmino Acid Sequence-
dc.subject.MESHBRCA2 Protein-
dc.subject.MESHDNA-Binding Proteins-
dc.subject.MESHGene Library-
dc.subject.MESHGenetic Vectors-
dc.subject.MESHHela Cells-
dc.subject.MESHHumans-
dc.subject.MESHKinesin-
dc.subject.MESHLeucine Zippers-
dc.subject.MESHMolecular Sequence Data-
dc.subject.MESHNeoplasm Proteins-
dc.subject.MESHPeptides-
dc.subject.MESHProtein Binding-
dc.subject.MESHProtein Interaction Mapping-
dc.subject.MESHProtein Structure, Tertiary-
dc.subject.MESHSaccharomyces cerevisiae-
dc.subject.MESHTumor Cells, Cultured-
dc.subject.MESHTwo-Hybrid System Techniques-
dc.titleAssociation of human kinesin superfamily protein member 4 with BRCA2-associated factor 35.-
dc.typeArticle-
dc.identifier.pmid12809554-
dc.identifier.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1223617/-
dc.contributor.affiliatedAuthor이, 영미-
dc.contributor.affiliatedAuthor김, 완기-
dc.type.localJournal Papers-
dc.identifier.doi10.1042/BJ20030452-
dc.citation.titleThe Biochemical journal-
dc.citation.volume374-
dc.citation.numberPt 2-
dc.citation.date2003-
dc.citation.startPage497-
dc.citation.endPage503-
dc.identifier.bibliographicCitationThe Biochemical journal, 374(Pt 2). : 497-503, 2003-
dc.identifier.eissn1470-8728-
dc.relation.journalidJ002646021-
Appears in Collections:
Journal Papers > Research Organization > Institute for Medical Sciences
Journal Papers > School of Medicine / Graduate School of Medicine > Pharmacology
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