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Transforming growth factor-beta-inducible gene-h3 (beta(ig)-h3) promotes cell adhesion of human astrocytoma cells in vitro: implication of alpha6beta4 integrin.

Kim, MO; Yun, SJ; Kim, IS; Sohn, S; Lee, EH
Neuroscience letters, 336(2):93-96, 2003
Journal Title
Neuroscience letters
Beta(ig)-h3 is a secretory protein that is induced by transforming growth factor (TGF)-beta. We have recently found that beta(ig)-h3 expression is induced in cultured astrocytes by TGF-beta1 and in rat cerebral cortex by stab wound. The purpose of this study was to examine the effect of the secreted beta(ig)-h3 on cell adhesion of astrocytes and the underlying mechanisms. When U87 human astrocytoma cells were seeded on dishes coated with recombinant beta(ig)-h3, cell adhesion was significantly enhanced. Blocking experiments using various antibodies to the integrin subunit suggested alpha6beta4 integrin could be involved in the beta(ig)-h3-mediated astrocyte cell adhesion. Cell adhesion to beta(ig)-h3 substrate was substantially blocked by preincubation with the inhibitor to the src kinase. When cells were plated on beta(ig)-h3-coated dishes, tyrosine phosphorylation of focal adhesion kinase was prominently increased within 20 min in a beta4 integrin-dependent manner. The results suggest that alpha6beta4 integrin-mediated interactions of astrocytes with beta(ig)-h3 transduce intracellular signals through the focal adhesion proteins, which may regulate certain aspects of astrocyte response to brain injury.
MeSH terms
Astrocytoma/metabolism*Astrocytoma/physiopathologyCell Adhesion/drug effectsCell Adhesion/genetics*Cytoskeletal Proteins/metabolismDose-Response Relationship, DrugExtracellular Matrix Proteins*HumansIntegrin alpha6beta1/metabolism*Neoplasm Proteins/administration & dosageNeoplasm Proteins/metabolism*Reproducibility of ResultsSensitivity and SpecificityTransforming Growth Factor beta*Tumor Cells, Culturedsrc-Family Kinases/metabolism
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
AJOU Authors
손, 성향
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