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Characterization and gene cloning of monoclonal antibody specific for the hepatitis B virus X protein.

Authors
Park, OY; Jin, YH; Lee, M; Shin, HJ; Kim, HI; Cho, H; Yun, CW; Youn, JK; Park, S
Citation
Hybridoma, 19(1):73-80, 2000
Journal Title
Hybridoma
ISSN
0272-457X
Abstract
The hepatis B virus X protein (HBx) has been thought to be implicated in the development of hepatocellular carcinoma. Although many functions of HBx have been reported, it is not clear which of HBx functions is important in hepatocellular carcinogenesis. To study HBx function, we produced a monoclonal anti-HBx Ab secreted by hybridoma cell clone H7 and mapped its epitope to a region of HBx between amino acids 29 and 48 by Western blot with truncated forms of HBx and by enzyme-linked immunoadsorbent assay (ELISA) with synthetic HBx peptides. The variable regions of H7 anti-HBx Ab were cloned by polymerase chain reaction using the degenerate-primers and by the 5' rapid amplification-cDNA end method. The sequence analyses revealed that the variable gene segments of the heavy and light chains are the members of mouse heavy chain variable gene 1 family and kappa light chain variable gene 2 family, respectively. In addition, J(H)2 or Jkappa4 gene segment at the end of the heavy-chain or light-chain variable region and DSP2.x gene segment in the CDR 3 of heavy chain were identified.
MeSH terms
Amino Acid SequenceAnimalsAntibodies, Monoclonal/biosynthesis/*chemistry/*geneticsAntibody SpecificityAntigen-Antibody ReactionsBase SequenceCloning, MolecularEpitope MappingHepatitis B Antibodies/biosynthesis/*chemistry/*geneticsHybridomasImmunoglobulin Variable Region/geneticsLiver/immunology/metabolismMaleMiceMice, Inbred BALB CMolecular Sequence DataTrans-Activators/*immunology
DOI
10.1089/027245700315815
PMID
10768843
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
Journal Papers > School of Medicine / Graduate School of Medicine > Gastroenterology
Journal Papers > School of Medicine / Graduate School of Medicine > Biochemistry & Molecular Biology
AJOU Authors
신, 호준김, 형일조, 혜성윤, 정구박, 선
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