Preparation and release behavior of ipriflavone-loaded PLGA microsphere for tissue engineered bone.
So, JW; Jang, JW; Kim, SH; Kim, GA; Choi, JH; Rhee, JM; Son, YS; Min, BH; Khang, G
POLYMER(KOREA), 33(1):26-32, 2009
The aim of this research was to prepare microparticulate systems based on poly (lactide- co-glycolide) (PLGA) for the local release of ipriflavone in order to reduce bone loss. We developed the IP loaded PLGA microspheres using relatively simple oil-in-water (O/W) solvent evaporation method. HPLC was used to perform the in vitro release test of IP and morphology of cell attached on the microspheres was investigated using SEM. Cytotoxicity was assayed by cell counting kit-8 (CCK-8) test. Osteogenic differential cells were analyzed by ALP activity. Through RT-PCR analysis, we observed osteocalcin, ALP, and Type I collagen mRNA expression. The release of IP in vitro was more prolonged over 42 days and IP/PLGA microspheres showed the improvement on the cell proliferation, ALP activity and RT-PCR comparing with control (only PLGA). This initial research will be used to direct future work involved in developing this composite injectable bone tissue engineering system.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
Total Visit :3,477,231
Total Download :1,455,792
Today View :1,659
Ajou University Medical Information & Media Center 164 Worldcup-ro Yeongtong-gu Suwon 16499 Korea / TEL : 031-219-5312 / FAX : 031-219-5314 Copyright (c) Ajou University Medical Information & Media Center All Rights Reserved. AJOU Open Repository는 국립중앙도서관 OAK 보급사업으로 구축되었습니다.