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Over-expression of ERT(ESX/ESE-1/ELF3), an ets-related transcription factor, induces endogenous TGF-beta type II receptor expression and restores the TGF-beta signaling pathway in Hs578t human breast cancer cells.

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dc.contributor.authorChang, J-
dc.contributor.authorLee, C-
dc.contributor.authorHahm, KB-
dc.contributor.authorYi, Y-
dc.contributor.authorChoi, SG-
dc.contributor.authorKim, SJ-
dc.date.accessioned2011-07-28-
dc.date.available2011-07-28-
dc.date.issued2000-
dc.identifier.issn0950-9232-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/3641-
dc.description.abstractThe epithelium-specific transcription factor, ERT/ESX/ESE-1/ELF3, binds to the TGF-beta RII promoter in a sequence specific manner and regulates its expression. In this study, we investigated whether ERT could regulate endogenous TGF-beta RII expression in Hs578t breast cancer cells. Analyses of the Hs578t parental cell line revealed low RII mRNA expression and resistance to the growth inhibitory effects of TGF-beta. Infection of this cell line with a retroviral construct expressing ERT induced higher levels of endogenous RII mRNA expression and protein expression relative to cells infected with chloramphenicol acetyltransferase (CATneo) as a control. Relative to control cells, the ERTneo-expressing Hs578t cells show approximately a 50% reduction in cell growth in the presence of exogenous TGF-beta1, as well as a fourfold higher induction of activation in transient transfection assays using the 3TP-luciferase reporter construct. When transplanted into athymic mice, ERT-expressing Hs578t cells showed decreased and delayed tumorigenicity compared with control cells. This data strongly suggests that ERT plays an important role as a transcriptional activator of TGF-beta RII expression, and that deregulated ERT expression may play a critical role in rendering Hs578t human breast cancer cells insensitive to TGF-beta's growth inhibitory effects.-
dc.language.isoen-
dc.subject.MESHAnimals-
dc.subject.MESHBreast Neoplasms-
dc.subject.MESHDNA-Binding Proteins-
dc.subject.MESHFemale-
dc.subject.MESHHumans-
dc.subject.MESHMice-
dc.subject.MESHMice, Nude-
dc.subject.MESHProtein-Serine-Threonine Kinases-
dc.subject.MESHProto-Oncogene Proteins-
dc.subject.MESHReceptors, Transforming Growth Factor beta-
dc.subject.MESHTrans-Activators-
dc.subject.MESHTranscription Factors-
dc.subject.MESHTransforming Growth Factor beta-
dc.titleOver-expression of ERT(ESX/ESE-1/ELF3), an ets-related transcription factor, induces endogenous TGF-beta type II receptor expression and restores the TGF-beta signaling pathway in Hs578t human breast cancer cells.-
dc.typeArticle-
dc.identifier.pmid10644990-
dc.contributor.affiliatedAuthor함, 기백-
dc.type.localJournal Papers-
dc.identifier.doi10.1038/sj.onc.1203252-
dc.citation.titleOncogene-
dc.citation.volume19-
dc.citation.number1-
dc.citation.date2000-
dc.citation.startPage151-
dc.citation.endPage154-
dc.identifier.bibliographicCitationOncogene, 19(1). : 151-154, 2000-
dc.identifier.eissn1476-5594-
dc.relation.journalidJ009509232-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Gastroenterology
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