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Grain dust induces IL-8 production from bronchial epithelial cells: effect on neutrophil recruitment.

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dc.contributor.authorPark, HS-
dc.contributor.authorSuh, JH-
dc.contributor.authorKim, SS-
dc.contributor.authorKwon, OJ-
dc.date.accessioned2011-07-28T02:13:31Z-
dc.date.available2011-07-28T02:13:31Z-
dc.date.issued2000-
dc.identifier.issn1081-1206-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/3652-
dc.description.abstractBACKGROUND: There have been several investigations suggesting an involvement of activated neutrophils in the development of grain dust (GD)-induced occupational asthma. Interleukin-8 in the sputa from GD-induced asthmatic patients increased significantly after the exposure to GD.



OBJECTIVE: To confirm IL-8 production from bronchial epithelial cells when exposed to GD, and to evaluate the role of IL-8 on neutrophil recruitment.



MATERIALS AND METHOD: We cultured Beas-2B, a bronchial epithelial cell line. To observe GD-induced responses, four different concentrations ranging from 1 to 200 microg/mL of GD were incubated for 24 hours and compared with those without incubation of GD. To evaluate the effect of pro-inflammatory cytokines on IL-8 production and neutrophil chemotaxis, epithelial cells were incubated with peripheral blood mononuclear cell (PBMC) culture supernatant derived from subjects with GD-induced asthma exposed to 10 microg/mL of GD, and then compared with those without addition of PBMC supernatant. The level of released IL-8 in the supernatant was measured by enzyme-linked immunosorbent assay. Neutrophil chemotactic activity of the culture supernatant was determined by modified Boyden chamber method.



RESULTS: Interleukin-8 production and neutrophil chemotactic activity from bronchial epithelial cells significantly increased with additions of GD in a dose-dependent manner (P < .05, respectively), and were significantly augmented with additions of PBMC supernatant (P < .05, respectively) at each concentration. Close correlation was noted between neutrophil chemotactic activity and IL-8 level (r = 0.87, P < .05). Compared with the untreated sample, pre-treatment of anti-IL-8 antibody induced a significant suppression (up to 67.2%) of neutrophil chemotactic activity in a dose-dependent manner.



CONCLUSION: These results suggest that IL-8 produced from bronchial epithelial cells may be a major cytokine, which induces neutrophil migration into the airways when exposed to GD.
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dc.language.isoen-
dc.subject.MESHAntibodies-
dc.subject.MESHBronchi-
dc.subject.MESHCereals-
dc.subject.MESHChemotaxis, Leukocyte-
dc.subject.MESHDust-
dc.subject.MESHEpithelial Cells-
dc.subject.MESHHumans-
dc.subject.MESHInterleukin-8-
dc.subject.MESHLeukocytes, Mononuclear-
dc.subject.MESHNeutrophils-
dc.titleGrain dust induces IL-8 production from bronchial epithelial cells: effect on neutrophil recruitment.-
dc.typeArticle-
dc.identifier.pmid10875492-
dc.identifier.urlhttp://linkinghub.elsevier.com/retrieve/pii/S1081-1206(10)62414-3-
dc.contributor.affiliatedAuthor박, 해심-
dc.contributor.affiliatedAuthor김, 선신-
dc.type.localJournal Papers-
dc.identifier.doi10.1016/S1081-1206(10)62414-3-
dc.citation.titleAnnals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology-
dc.citation.volume84-
dc.citation.number6-
dc.citation.date2000-
dc.citation.startPage623-
dc.citation.endPage627-
dc.identifier.bibliographicCitationAnnals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology, 84(6). : 623-627, 2000-
dc.identifier.eissn1534-4436-
dc.relation.journalidJ010811206-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Allergy
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