18 173

Cited 304 times in

A murine model of peanut anaphylaxis: T- and B-cell responses to a major peanut allergen mimic human responses.

Authors
Li, XM; Serebrisky, D; Lee, SY; Huang, CK; Bardina, L; Schofield, BH; Stanley, JS; Burks, AW; Bannon, GA; Sampson, HA
Citation
The Journal of allergy and clinical immunology, 106(1Pt1):150-158, 2000
Journal Title
The Journal of allergy and clinical immunology
ISSN
0091-67491097-6825
Abstract
BACKGROUND: Peanut allergy affects 0.6% of the US population. At the present time, allergen avoidance is the only therapeutic option. Animal models of food-induced anaphylaxis would facilitate attempts to design novel immunotherapeutic strategies for the treatment of peanut allergy.



OBJECTIVE: The purpose of this study was to develop a murine model of IgE-mediated peanut hypersensitivity that closely mimics human peanut allergy.



METHODS: C3H/HeJ mice sensitized orally with freshly ground whole peanut and cholera toxin as adjuvant were challenged orally 3 and 5 weeks later with crude peanut extract. Anaphylactic reactions were determined. T- and B-cell responses to Ara h 1 and Ara h 2, the major peanut allergens, were characterized by evaluating splenocyte proliferative responses and IgE antibody concentrations. Furthermore, IgE antibodies in the sera of patients with peanut allergy and mice were compared for antibody binding to Ara h 2 isoforms and allergenic epitopes.



RESULTS: Peanut-specific IgE was induced by oral peanut sensitization, and hypersensitivity reactions were provoked by feeding peanut to sensitized mice. The symptoms were similar to those seen in human subjects. Ara h 1- and Ara h 2-specific antibodies were present in the sera of mice with peanut allergy. Furthermore, these Ara h 2-specific IgE antibodies bound the same Ara h 2 isoforms and major allergenic epitopes as antibodies in the sera of human subjects with peanut allergy. Splenocytes from mice with peanut allergy exhibited proliferative responses to Ara h 1 and Ara h 2.



CONCLUSION: This murine model of peanut allergy mimics the clinical and immunologic characteristics of peanut allergy in human subjects and should be a useful tool for developing immunotherapeutic approaches for the treatment of peanut allergy.
MeSH terms
2S Albumins, PlantAllergens/*adverse effects/immunologyAnaphylaxis/*chemically induced/immunologyAnimalsAntigens, PlantArachis hypogaea/*adverse effects/immunologyB-Lymphocytes/*immunology*Disease Models, AnimalFemaleFood Hypersensitivity/immunologyGlycoproteins/adverse effects/immunologyHumansImmunoglobulin E/immunologyMiceMice, Inbred C3HPlant Proteins/adverse effects/immunologySkin TestsT-Lymphocytes/*immunology
DOI
10.1067/mai.2000.107395
PMID
10887318
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Pediatrics & Adolescent Medicine
AJOU Authors
이, 수영
Full Text Link
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse