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CpG oligodeoxynucleotides can reverse Th2-associated allergic airway responses and alter the B7.1/B7.2 expression in a murine model of asthma.

Authors
Serebrisky, D; Teper, AA; Huang, CK; Lee, SY; Zhang, TF; Schofield, BH; Kattan, M; Sampson, HA; Li, XM
Citation
Journal of immunology (Baltimore, Md. : 1950), 165(10):5906-5912, 2000
Journal Title
Journal of immunology (Baltimore, Md. : 1950)
ISSN
0022-17671550-6606
Abstract
CpG oligodeoxynucleotides (CpG-ODN) administered during Ag sensitization or before Ag challenge can inhibit allergic pulmonary inflammation and airway hyperreactivity in murine models of asthma. In this study, we investigated whether CpG-ODN can reverse an ongoing allergic pulmonary reaction in a mouse model of asthma. AKR mice were sensitized with conalbumin followed by two intratracheal challenges at weekly intervals. CpG-ODN was administered 24 h after the first Ag challenge. CpG-ODN administration reduced Ag-specific IgE levels, bronchoalveolar lavage fluid eosinophils, mucus production, and airway hyperreactivity. We found that postchallenge CpG-ODN treatment significantly increased IFN-gamma concentrations and decreased IL-13, IL-4, and IL-5 concentrations in bronchoalveolar lavage fluids and spleen cell culture supernatants. Postchallenge CpG-ODN treatment also increased B7.1 mRNA expression and decreased B7.2 mRNA expression in lung tissues. These results suggest that CpG-ODN may have potential for treatment of allergic asthma by suppressing Th2 responses during IgE-dependent allergic airway reactions. The down-regulation of Th2 responses by CPG-ODN may be associated with regulation of the costimulatory factors B7.1 and B7.2.
MeSH terms
Adjuvants, Immunologic/administration & dosage/*pharmacologyAnimalsAntigens, CD/*biosynthesis/geneticsAntigens, CD80/*biosynthesis/geneticsAntigens, CD86Asthma/*immunology/pathologyBronchial Hyperreactivity/*immunologyCells, CulturedConalbumin/administration & dosage/immunologyCpG Islands/*immunologyDisease Models, AnimalDown-Regulation/immunologyHyperplasiaImmunoglobulins/biosynthesisInjections, IntraperitonealInterferon-gamma/antagonists & inhibitors/biosynthesisInterleukin-13/biosynthesisInterleukin-4/biosynthesisInterleukin-5/biosynthesisMaleMembrane Glycoproteins/*biosynthesis/geneticsMiceMice, Inbred AKROligodeoxyribonucleotides/administration & dosage/*pharmacologyRNA, Messenger/biosynthesisRespiratory Mucosa/immunology/pathologyTh2 Cells/*immunologyUp-Regulation/immunology
PMID
11067952
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Journal Papers > School of Medicine / Graduate School of Medicine > Pediatrics & Adolescent Medicine
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