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Overexpression of BETA2/NeuroD induces neurite outgrowth in F11 neuroblastoma cells.

Cho, JH; Kwon, IS; Kim, S; Ghil, SH; Tsai, MJ; Kim, YS; Lee, YD; Suh-Kim, H
Journal of neurochemistry, 77(1):103-109, 2001
Journal Title
Journal of neurochemistry
BETA2/NeuroD, a basic helix-loop-helix (bHLH) transcription factor, has been shown to play important roles in the development of the nervous system and the maintenance and formation of pancreatic and enteroendocrine cells. The gain of function of BETA2/NeuroD in neurogenesis has been shown in Xenopus embryos. In this study, we investigated the neurogenic potential of BETA2/NeuroD using neuroblastoma cell line, F11, which could be induced to differentiate into neurons in the presence of cAMP. To induce or block the expression of BETA2/NeuroD, expression vectors for the full-length and a C-terminal deletion mutant of BETA2 were constructed and their transactivation potential was verified using reporter genes containing the insulin promoter sequences. Overexpression of BETA2 with full-length construct induced neurite outgrowth in F11 cells in the absence of cAMP. In contrast, the C-terminal deletion mutant, BETA2(1--233), which has dominant negative activity, inhibited neurite outgrowth induced by cAMP in F11 cells. These results indicate that BETA2/NeuroD plays an important role in terminal differentiation of neuroblastoma cells. They also imply that BETA2/NeuroD or related bHLH factors plays an essential role for differentiation of F11 neuroblastoma cells.
MeSH terms
AnimalsBasic Helix-Loop-Helix Transcription FactorsCell Differentiation/drug effectsCell Differentiation/genetics*Cyclic AMP/pharmacologyGene Expression Regulation, Neoplastic/drug effectsGene Expression Regulation, Neoplastic/geneticsGenes, DominantGenes, ReporterGenetic Vectors/geneticsGenetic Vectors/metabolismGenetic Vectors/pharmacologyHelix-Loop-Helix MotifsInsulin/geneticsMutagenesis, Site-DirectedNerve Tissue Proteins/biosynthesis*Nerve Tissue Proteins/geneticsNerve Tissue Proteins/pharmacologyNeurites/drug effectsNeurites/metabolism*Neuroblastoma/geneticsNeuroblastoma/metabolism*Promoter Regions, Genetic/drug effectsRatsSequence DeletionTransfectionTumor Cells, Cultured
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Journal Papers > School of Medicine / Graduate School of Medicine > Anatomy
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