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Rutaecarpine, a quinazolinocarboline alkaloid, inhibits prostaglandin production in RAW264.7 macrophages.

Authors
Woo, HG; Lee, CH; Noh, MS; Lee, JJ; Jung, YS; Baik, EJ; Moon, CH; Lee, SH
Citation
Planta medica, 67(6):505-509, 2001
Journal Title
Planta medica
ISSN
0032-09431439-0221
Abstract
In order to delineate the mechanism involved in the anti-inflammatory activity of rutaecarpine, its effects on the production of prostaglandin (PG) and therein involved enzymes were examined. Rutaecarpine reduced the production of PGE(2) in RAW264.7 cells treated with lipopolysaccharide (LPS) in a dose dependent manner when added to the culture media at the time of stimulation. However, the inhibition of total cellular cyclooxygenase (COX) activity under the same experimental condition was observed only at high concentrations of rutaecarpine. Rutaecarpine did not affected the levels of COX-2 mRNA and protein in macrophages stimulated with LPS. Calcium ionophore A23187 induced-PG production and [(3)H]-arachidonic acid release were significantly decreased by the pretreatment of rutaecarpine for 30 minutes. With the same treatment schedule, however, rutaecarpine failed to alter the activities of cellular COX-1 and COX-2. Collectively, our data suggest that anti-inflammatory effect of rutaecarpine is, at least in part, ascribed to the diminution of PG production through inhibition of arachidonic acid release albeit the nature of its effects on PLA(2) activity remains to be elaborated.
MeSH terms
Alkaloids/pharmacology*Angiosperms/chemistryAnimalsArachidonic Acid/metabolismCell SurvivalCells, CulturedCyclooxygenase 1Cyclooxygenase 2Cyclooxygenase 2 InhibitorsCyclooxygenase Inhibitors/pharmacology*Fruit/chemistryIndole AlkaloidsIsoenzymes/antagonists & inhibitorsMacrophages/drug effects*Plants, Medicinal
DOI
10.1055/s-2001-16479
PMID
11509968
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Physiology
AJOU Authors
정, 이숙백, 은주문, 창현이, 수환
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