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Purification and characterization of protein methylase II from Helicobacter pylori.

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dc.contributor.authorKim, YM-
dc.contributor.authorAhn, SH-
dc.contributor.authorSeo, DW-
dc.contributor.authorKim, YK-
dc.contributor.authorHan, JW-
dc.contributor.authorHong, S-
dc.contributor.authorKim, S-
dc.contributor.authorPaik, WK-
dc.contributor.authorLee, HW-
dc.date.accessioned2011-08-04T05:03:34Z-
dc.date.available2011-08-04T05:03:34Z-
dc.date.issued2001-
dc.identifier.issn0378-1097-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/3696-
dc.description.abstractProtein methylase II (AdoMet:protein-carboxyl O-methyltransferase, EC 2.1.1.24) was identified and purified 115-fold from Helicobacter pylori through Q-Sepharose ion exchange column, AdoHcy-Sepharose 4B column, and Superdex 200 HR column chromatography using FPLC. The purified preparation showed two protein bands of about 78 kDa and 29 kDa molecular mass on SDS-PAGE. On non-denaturing gel electrophoresis, the enzyme migrated as a single band with a molecular mass of 410 kDa. In addition, MALDI-TOF-MS analysis and Superdex 200 HR column chromatography of the purified enzyme showed a major mass signal with molecular mass values of 425 kDa and 430 kDa, respectively. Therefore, the above results led us to suggest that protein methylase II purified from H. pylori is composed of four heterodimers with 425 kDa (4x(78+29)=428 kDa). This magnitude of molecular mass is unusual for protein methylases II so far reported. The enzyme has an optimal pH of 6.0, a K(m) value of 5.0x10(-6) M for S-adenosyl-L-methionine and a V(max) of 205 pmol methyl-(14)C transferred min(-1) mg(-1) protein.-
dc.language.isoen-
dc.subject.MESHAmino Acid Sequence-
dc.subject.MESHHelicobacter Infections-
dc.subject.MESHHelicobacter pylori-
dc.subject.MESHHumans-
dc.subject.MESHMolecular Sequence Data-
dc.subject.MESHProtein O-Methyltransferase-
dc.subject.MESHSubstrate Specificity-
dc.titlePurification and characterization of protein methylase II from Helicobacter pylori.-
dc.typeArticle-
dc.identifier.pmid11166995-
dc.contributor.affiliatedAuthor백, 운기-
dc.type.localJournal Papers-
dc.citation.titleFEMS microbiology letters-
dc.citation.volume195-
dc.citation.number1-
dc.citation.date2001-
dc.citation.startPage53-
dc.citation.endPage58-
dc.identifier.bibliographicCitationFEMS microbiology letters, 195(1). : 53-58, 2001-
dc.identifier.eissn1574-6968-
dc.relation.journalidJ003781097-
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Journal Papers > School of Medicine / Graduate School of Medicine > Biochemistry & Molecular Biology
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