Cited 0 times in Scipus Cited Count

Behavioral differences between donor site-matched adult and neonatal melanocytes in culture.

DC Field Value Language
dc.contributor.authorKim, NS-
dc.contributor.authorCho, JH-
dc.contributor.authorKang, WH-
dc.date.accessioned2011-08-09T05:43:49Z-
dc.date.available2011-08-09T05:43:49Z-
dc.date.issued2000-
dc.identifier.issn0340-3696-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/3711-
dc.description.abstractLittle is known about the biologic behaviors of cultured melanocytes in relation to donor age. To investigate age-dependent differences, neonatal and adult melanocytes were isolated from the same anatomical site, the foreskin, and cultured in the same growth medium supplemented with cAMP inducers (choleratoxin and 3-isobutyl-methylxanthine). The morphology, melanin content, pattern of melanosome distribution, and growth rate were then compared. Neonatal melanocytes were bipolar in appearance, whereas adult melanocytes were highly dendritic in appearance. Image analysis showed that adult melanocytes were larger and longer, and had a greater number of dendrites than neonatal melanocytes. When the growth medium was replaced by a medium without cAMP inducers, adult melanocytes showed a change in their morphology from dendritic to spindle-shaped, while the morphology of neonatal melanocytes remained unchanged. Melanosomes of adult melanocytes were distributed singly along the dendrites, and extracellular secretion of melanosomes was also found. In contrast, melanosomes of neonatal melanocytes were aggregated near the nuclei. No age-dependent differences in melanin content and growth rate were noted in the donor site-matched cultured melanocytes. These results suggest that donor age is one of the factors involved in determining melanocyte dendricity and melanosome distribution, and that increased dendricity of adult melanocytes is due to increased sensitivity to cAMP inducers. In addition, the adult melanocytes established in our culture system, which resembled dendritic melanocytes in vivo, could be considered a desirable model for studying the mechanisms of adult-onset hyperpigmentary disorders and melanogenesis.-
dc.language.isoen-
dc.subject.MESH1-Methyl-3-isobutylxanthine-
dc.subject.MESHAdolescent-
dc.subject.MESHAdult-
dc.subject.MESHCell Division-
dc.subject.MESHCell Size-
dc.subject.MESHCells, Cultured-
dc.subject.MESHCholera Toxin-
dc.subject.MESHCulture Media-
dc.subject.MESHHumans-
dc.subject.MESHInfant, Newborn-
dc.subject.MESHMale-
dc.subject.MESHMelanins-
dc.subject.MESHMelanocytes-
dc.subject.MESHMelanosomes-
dc.subject.MESHSpectrophotometry-
dc.subject.MESHTime Factors-
dc.titleBehavioral differences between donor site-matched adult and neonatal melanocytes in culture.-
dc.typeArticle-
dc.identifier.pmid10867811-
dc.identifier.urlhttps://link.springer.com/content/pdf/10.1007/s004030050480.pdf-
dc.contributor.affiliatedAuthor강, 원형-
dc.type.localJournal Papers-
dc.identifier.doi10.1007/s004030050480-
dc.citation.titleArchives of dermatological research-
dc.citation.volume292-
dc.citation.number5-
dc.citation.date2000-
dc.citation.startPage233-
dc.citation.endPage239-
dc.identifier.bibliographicCitationArchives of dermatological research, 292(5). : 233-239, 2000-
dc.identifier.eissn1432-069X-
dc.relation.journalidJ003403696-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Dermatology
Files in This Item:
There are no files associated with this item.

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse