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Production and characterization of immortalized rat hepatocytes secreting hepatocyte growth factor/scatter factor.

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dc.contributor.authorLee, JH-
dc.contributor.authorKim, WH-
dc.contributor.authorPark, H-
dc.contributor.authorYun, C-
dc.contributor.authorKim, BH-
dc.contributor.authorKwak, SJ-
dc.contributor.authorCho, H-
dc.contributor.authorKim, MW-
dc.date.accessioned2011-08-10T05:25:27Z-
dc.date.available2011-08-10T05:25:27Z-
dc.date.issued2000-
dc.identifier.issn0172-6390-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/3727-
dc.description.abstractBACKGROUND/AIMS: Hepatocyte transplantation is a recently attractive field in the treatment of liver failure and enzyme-deficient diseases. However, procurement of sufficient quantities of hepatocytes is almost impossible. We attempted to create a hepatocyte cell line that could be used for hepatocyte transplantation.



METHODOLOGY: L2A2 is a conditionally immortalized rat hepatocyte cell line produced by transfection of temperature-sensitive simian virus T antigen to the hepatocytes in the Lewis rat. Hepatocyte Growth Factor/Scatter Factor (HGF/SF)-secreting L2A2 cells, designated as SF-21, was produced by transfecting human HGF/SF cDNA into L2A2 cells.



RESULTS: This cell line was able to produce HGF/SF at the rate of 5-10 ng/10(6) cells/24 hrs, and the recombinant HGF/SF was of the expected size and was functionally active in that it could scatter Madin-Darby canine kidney cells. The SF-21 cells grew faster than its parental cell clone, and survived and proliferated at 37 degrees C in vitro. Also, the SF-21 cells were able to survive and proliferate when transplanted into the spleen of syngeneic rat, and expressed glucose-6-phosphatase.



CONCLUSIONS: These HGF/SF-secreting hepatocytes can be used as a model system to test a feasibility of using genetically engineered hepatocyte cell line for hepatocyte transplantation in the rat.
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dc.language.isoen-
dc.subject.MESHAnimals-
dc.subject.MESHBlotting, Western-
dc.subject.MESHCell Culture Techniques-
dc.subject.MESHCell Line-
dc.subject.MESHHepatocyte Growth Factor-
dc.subject.MESHHepatocytes-
dc.subject.MESHImmunohistochemistry-
dc.subject.MESHMale-
dc.subject.MESHRats-
dc.subject.MESHRats, Inbred Lew-
dc.subject.MESHTransfection-
dc.titleProduction and characterization of immortalized rat hepatocytes secreting hepatocyte growth factor/scatter factor.-
dc.typeArticle-
dc.identifier.pmid11020861-
dc.contributor.affiliatedAuthor이, 재호-
dc.contributor.affiliatedAuthor김, 욱환-
dc.contributor.affiliatedAuthor조, 혜성-
dc.contributor.affiliatedAuthor김, 명욱-
dc.type.localJournal Papers-
dc.citation.titleHepato-gastroenterology-
dc.citation.volume47-
dc.citation.number34-
dc.citation.date2000-
dc.citation.startPage978-
dc.citation.endPage983-
dc.identifier.bibliographicCitationHepato-gastroenterology, 47(34). : 978-983, 2000-
dc.relation.journalidJ001726390-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Biochemistry & Molecular Biology
Journal Papers > School of Medicine / Graduate School of Medicine > Surgery
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