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Immunodominant antigens in Naegleria fowleri excretory--secretory proteins were potential pathogenic factors.

Authors
Kim, JH; Yang, AH; Sohn, HJ; Kim, D; Song, KJ; Shin, HJ
Citation
Parasitology research, 105(6):1675-1681, 2009
Journal Title
Parasitology research
ISSN
0932-01131432-1955
Abstract
Naegleria fowleri, a ubiquitous pathogenic free-living amoeba, is the most virulent species and causes primary amoebic meningoencephalitis in laboratory animals and humans. The parasite secretes various inducing molecules as biological responses, which are thought to be involved in pathophysiological and immunological events during infection. To investigate what molecules of N. fowleri excretory-secretory proteins (ESPs) are related with amoebic pathogenicity, N. fowleri ESPs fractionated by two-dimensional electrophoresis were reacted with N. fowleri infection or immune sera. To identify immunodominant ESPs, six major protein spots were selected and analyzed by N-terminal sequencing. Finally, six proteins, 58, 40, 24, 21, 18, and 16 kDa of molecular weight, were partially cloned and matched with reference proteins as follow: 58 kDa of exendin-3 precursor, 40 kDa of secretory lipase, 24 kDa of cathepsin B-like proteases and cysteine protease, 21 kDa of cathepsin B, 18 kDa of peroxiredoxin, and 16 kDa of thrombin receptor, respectively. These results suggest that N. fowleri ESPs contained important proteins, which may play an important role in the pathogenicity of N. fowleri.
MeSH terms
AnimalsAntibodies, Protozoan/immunologyAntigens, Protozoan/chemistryAntigens, Protozoan/immunology*Antigens, Protozoan/isolation & purificationCloning, MolecularElectrophoresis, Gel, Two-DimensionalFemaleImmunoblottingImmunodominant Epitopes/chemistryImmunodominant Epitopes/immunology*Immunodominant Epitopes/isolation & purificationMiceMice, Inbred BALB CMolecular WeightNaegleria fowleri/immunology*Proteome/analysisProtozoan Proteins/chemistryProtozoan Proteins/immunology*Protozoan Proteins/isolation & purificationVirulence Factors/immunology
DOI
10.1007/s00436-009-1610-y
PMID
19756751
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
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