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Acute application of interleukin-1beta induces Ca(2+) responses in human microglia.

Authors
Goghari, V; Franciosi, S; Kim, SU; Lee, YB; McLarnon, JG
Citation
Neuroscience letters, 281(2-3):83-86, 2000
Journal Title
Neuroscience letters
ISSN
0304-39401872-7972
Abstract
The effects of the pro-inflammatory cytokine interleukin-1-beta (IL-1beta) on levels of intracellular calcium [Ca(2+)](i) in cultured human microglia have been studied using the fluorescent Ca(2+) indicator fura-2. IL-1beta (2 ng/ml) caused a slow, progressive increase in [Ca(2+)](i) in standard Ca(2+)-containing physiological solution (PSS). A similar effect was observed in separate studies using Ca(2+)-free PSS, however, the mean rate of increase was significantly lower than that measured with PSS. Similar results were obtained in a separate protocol, where cells were exposed to both IL-1beta in Ca(2+)-free PSS and PSS. The slope of the IL-1beta induced increase of [Ca(2+)](i) in Ca(2+)-free PSS was not altered when adenosine triphosphate was added prior to application of the cytokine. These results suggest that IL-1beta-induced responses in human microglia involve both a Ca(2+) entry pathway and a mechanism of intracellular increase other than from IP(3)-sensitive stores.
MeSH terms
Calcium/*metabolismCells, CulturedEmbryo, MammalianHumansInterleukin-1/*metabolism/pharmacologyMicroglia/drug effects/*metabolism
PMID
10704748
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Neurology
Journal Papers > Research Organization > Institute for Medical Sciences
AJOU Authors
김, 승업이, 용범
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