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Synaptotagmin IV is an immediate early gene induced by depolarization in PC12 cells and in brain.

Authors
Vician, L; Lim, IK; Ferguson, G; Toccoq, G; Baudry, M; Herschman, HR
Citation
Proceedings of the National Academy of Sciences of the United States of America, 92(6):2164-2168, 1995
Journal Title
Proceedings of the National Academy of Sciences of the United States of America
ISSN
0027-84241091-6490
Abstract
Subtractive library construction and differential screening were used to identify a cDNA for a cell type-specific immediate early gene induced in rat PC12 pheochromocytoma cells. Sequencing identified the protein product of this gene as rat synaptotagmin IV (SytIV). Synaptotagmins are synaptic vesicle proteins thought to play a role in depolarization-induced, calcium-mediated exocytosis and neurotransmitter release. SytIV mRNA accumulation is transiently induced in PC12 cells by potassium depolarization, calcium ionophore, ATP, and forskolin. In contrast, growth factors and phorbol 12-myristate 13-acetate induce little or no SytIV mRNA accumulation. Kainic acid-induced seizures in rats are followed by accumulation of SytIV message in the hippocampus and piriform cortex. The SytIV gene may provide a direct link between depolarization-induced neuronal gene expression and subsequent modulation of synaptic structure and function.
MeSH terms
Adrenal Gland NeoplasmsAmino Acid SequenceAnimalsBase SequenceBrain/drug effects/*metabolism*Calcium-Binding ProteinsCell LineDNA PrimersForskolin/pharmacology*Gene Expression/drug effectsGene Library*Genes, Immediate-EarlyHippocampus/metabolismKainic Acid/pharmacologyMaleMembrane Glycoproteins/*biosynthesis/geneticsMembrane PotentialsMiceMolecular Sequence DataNerve Tissue Proteins/*biosynthesis/geneticsOligonucleotide ProbesOrgan SpecificityPC12 CellsPheochromocytomaPolymerase Chain ReactionPotassium/pharmacologyRNA, Messenger/analysis/biosynthesisRatsRats, Sprague-DawleySeizures/chemically induced/metabolismSequence Homology, Amino AcidSynaptotagminsTetradecanoylphorbol Acetate/pharmacology
PMID
7892240
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Biochemistry & Molecular Biology
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