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Blockade of glutamate receptors unmasks neuronal apoptosis after oxygen-glucose deprivation in vitro.

Authors
Gwag, BJ; Lobner, D; Koh, JY; Wie, MB; Choi, DW
Citation
Neuroscience, 68(3):615-619, 1995
Journal Title
Neuroscience
ISSN
0306-45221873-7544
Abstract
Mouse cortical cell cultures exposed to transient oxygen-glucose deprivation developed marked acute cell body swelling followed by neurodegeneration, consistent with necrosis-type death. This death was not attenuated by the protein synthesis inhibitor, cycloheximide, but was attenuated by addition of the N-methyl-D-asparate antagonist, MK-801 (dizocilpine maleate), and the alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid/kainate antagonist, 6-cyano-7-nitroquinoxaline-2,3-dione. If the deprivation insult was extended to overcome the protective effect of glutamate antagonists, neuronal death resulted that was associated with cell body shrinkage and DNA fragmentation, and was attenuated by cycloheximide. These data suggest that oxygen-glucose deprivation can induce in cortical neurons both excitotoxic necrosis, and apoptosis dependent on new macromolecule synthesis.
MeSH terms
6-Cyano-7-nitroquinoxaline-2,3-dione/pharmacologyAnimalsApoptosis/drug effects/*physiologyCell Hypoxia/*physiologyCerebral Cortex/cytologyCulture MediaCycloheximide/pharmacologyDizocilpine Maleate/pharmacologyExcitatory Amino Acid Antagonists/*pharmacologyExcitatory Amino Acids/toxicityGlucose/physiologyMiceNeurons/drug effects/*physiologyProtein Synthesis Inhibitors/pharmacologyReceptors, AMPA/antagonists & inhibitorsReceptors, Glutamate/*metabolismReceptors, N-Methyl-D-Aspartate/antagonists & inhibitors
PMID
8577361
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Pharmacology
AJOU Authors
곽, 병주
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