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Expression and function of outward K+ channels induced by lipopolysaccharide in microglia.

Pyo, H; Chung, S; Jou, I; Gwag, BJ; Joe, HY
Molecules and cells, 7(5):610-614, 1997
Journal Title
Molecules and cells
Cultured microglia exposed to lipopolysaccharide (LPS) express outward K+ currents. These currents are due to the expression of new K+ channels detected by antibodies against Kv1.5, a shaker type delayed rectifier. We examined whether the K+ currents are involved in microglial activation. The amount of nitrite, converted from nitric oxide (NO), was measured as an indication of microglial activation [Chao, C. C., Hu, S., Molitor, T. W., Shaskan, E. G., and Peterson, P. K. (1992) J. Immunol. 149, 2730-2741]. Nitrite was detected starting from 24 h after LPS treatment and continuously increased over 4 days. Contrary to this, in the presence of 4-aminopyridine (4-AP, 2 mM), a blocker of outward K+ currents, nitrite production was reduced to less than 50%. However, the treatment of 4-AP 24 h after LPS did not reduce nitrite production. Other K+ channel blockers having a less blocking effect on outward K+ currents, such as 5 mM tetraethylammonium (TEA) and 1 mM Cs+, had little effect. The present study shows that the outward K+ channels induced by LPS are immunologically related to Kv1.5 and also suggests that these channels are required for microglial activation, particularly for the initiation of the activation process.
MeSH terms
AnimalsAntibodiesCerebral CortexKv1.5 Potassium ChannelLipopolysaccharides/*pharmacologyMicroglia/chemistry/*drug effects/*metabolismNitric Oxide/antagonists & inhibitors/metabolismPotassium Channels/analysis/*biosynthesis/immunology/*physiology*Potassium Channels, Voltage-GatedRatsRats, Sprague-DawleyStaining and Labeling
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Pharmacology
AJOU Authors
주, 일로곽, 병주조, 은혜
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