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Colorimetric microwell plate hybridization assay for detection of amplified Mycobacterium tuberculosis DNA from sputum samples.

Authors
Cho, SN; van der Vilet, GM; Park, S; Baik, SH; Kim, SK; Chong, Y; Kolk, AH; Klatser, PR; Kim, JD
Citation
Journal of clinical microbiology, 33(3):752-754, 1995
Journal Title
Journal of clinical microbiology
ISSN
0095-11371098-660X
Abstract
We developed a colorimetric microwell plate hybridization assay (CoMPHA) for the specific detection of 5'-biotinylated amplified Mycobacterium tuberculosis DNA. The optical densities of the CoMPHA corresponded to the initial amounts of purified template DNA. Here, we show that the CoMPHA is useful in distinguishing the PCR-positive and PCR-negative samples.
MeSH terms
Base SequenceColorimetry/methodsDNA ProbesDNA, Viral/*analysisHumansMolecular Sequence DataMycobacterium tuberculosis/genetics/*isolation & purificationNucleic Acid HybridizationPolymerase Chain Reaction/methodsSputum/*virology
PMID
7751391
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
AJOU Authors
박, 선
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