Colorimetric microwell plate hybridization assay for detection of amplified Mycobacterium tuberculosis DNA from sputum samples.
Cho, SN; van der Vilet, GM; Park, S; Baik, SH; Kim, SK; Chong, Y; Kolk, AH; Klatser, PR; Kim, JD
Journal of clinical microbiology, 33(3):752-754, 1995
Journal of clinical microbiology
We developed a colorimetric microwell plate hybridization assay (CoMPHA) for the specific detection of 5'-biotinylated amplified Mycobacterium tuberculosis DNA. The optical densities of the CoMPHA corresponded to the initial amounts of purified template DNA. Here, we show that the CoMPHA is useful in distinguishing the PCR-positive and PCR-negative samples.
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