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GT1b ganglioside induces death of dopaminergic neurons in rat mesencephalic cultures.

Authors
Chung, ES; Joe, EH; Ryu, JK; Kim, J; Lee, YB; Cho, KG; Oh, YJ; Maeng, SH; Baik, HH; Kim, SU; Jin, BK
Citation
Neuroreport, 12(3):611-614, 2001
Journal Title
Neuroreport
ISSN
0959-49651473-558X
Abstract
We examined neurotoxicity of GT1b against dopaminergic neurons in vitro. Cultures of mesencephalic cells deprived of serum underwent the loss of 19% of tyrosine hydroxylase immunopositive (TH-ip) neurons. In cultures deprived of serum, treatment with 10-30 microg/ml GT1b attenuated the number of TH-ip neurons by 26-69%, respectively, compared to non-treated cultures. Intriguingly, cultures deprived of serum were more vulnerable to GT1b-induced neurotoxicity. Application of 60 microg/ml GT1b to cultures grown in serum containing media resulted in the loss of 26% of TH-ip neurons, similar to that (28%) observed in serum-deprived cultures treated with 10 microg/ml GT1b. Moreover, in our cultures, absence of nitric oxide (NO) production after GT1b treatment was obvious. The present results strongly suggest direct neurotoxic actions of GT1b against dopaminergic neurons regardless of NO.
MeSH terms
AnimalsCell Death/drug effects*Cells, CulturedDopamine/physiology*Gangliosides/toxicity*Mesencephalon/cytologyMicroglia/cytologyMicroglia/metabolismNeurons/cytology*Neurons/enzymologyNitric Oxide/metabolismRatsTyrosine 3-Monooxygenase/analysis
PMID
11234774
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Pharmacology
Journal Papers > School of Medicine / Graduate School of Medicine > Neurosurgery
Journal Papers > School of Medicine / Graduate School of Medicine > Neurology
Journal Papers > Research Organization > Institute for Medical Sciences
AJOU Authors
조, 은혜이, 용범조, 경기김, 승업진, 병관
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