58 114

Cited 0 times in

Methylmercury neurotoxicity in cultures of human neurons, astrocytes, neuroblastoma cells

Sanfeliu, C; Sebastia, J; Ki, SU
Neurotoxicology, 22(3):317-327, 2001
Journal Title
Neurotoxic effects of methylmercury, were investigated in vitro in primary cultures of human neurons and astrocytes isolatedfrom human fetal brain and in the human neuroblastoma cell line SH-SY5Y. The protection provided by agents with antioxidant properties was tested in these cultures to examine the oxidative stress mechanism of methylmercury poisoning. After 24 h of exposure to methylmercury, LC50 values were 6.5, 8.1 and 6.9 microM for human neurons, astrocytes and neuroblastoma cells, respectively, and the degree of cell damage increased at longer exposure times. Depletion of the cellular pool of reduced glutathione (GSH) by treatment with buthionine sulfoximine potentiated methylmercury cytotoxicity in all three cell types; neuroblastoma cells were the most sensitive. Addition of GSH extracellularly blocked methylmercury neurotoxicity in all cell types. The major beneficial effect of GSH could be attributed to its capacity to form conjugates with methylmercury, which reduces the availability of these organometallic molecules to the cells and facilitates their efflux. Cysteine protected astrocytes and neuroblastoma cells from methylmercury neurotoxicity, while selenite, Vitamin E and catalase produced some minor protective effects in three cell types, particularly in neurons. The present study showed that the human neural cells tested had differential responses to methylmercury: astrocytes were resistant to methylmercury neurotoxicity and neurons were more most responsive to protection afforded by antioxidants among the three cell types.
MeSH terms
Antioxidants/pharmacologyAstrocytes/drug effects*Cell Survival/drug effectsCells, Cultured/drug effectsDose-Response Relationship, DrugFetusGlutathione/pharmacologyHumansMethylmercury Compounds/toxicity*Neuroblastoma/pathology*Neurons/drug effects*Tumor Cells, Cultured/drug effects
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Neurology
AJOU Authors
김, 승업
Full Text Link
Files in This Item:
Full-Text Not Available.txtDownload
RIS (EndNote)
XLS (Excel)


해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.