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A novel ets-related transcription factor, ERT/ESX/ESE-1, regulates expression of the transforming growth factor-beta type II receptor.

Authors
Choi, SG; Yi, Y; Kim, YS; Kato, M; Chang, J; Chung, HW; Hahm, KB; Yang, HK; Rhee, HH; Bang, YJ; Kim, SJ
Citation
The Journal of biological chemistry, 273(1):110-117, 1998
Journal Title
The Journal of biological chemistry
ISSN
0021-92581083-351X
Abstract
A 2.5-kilobase cDNA clone that encodes a 371-amino acid novel transcription factor was isolated from a human placenta cDNA library using a yeast one-hybrid system. The novel ets-related transcription factor (ERT) showed a homology with the ETS DNA-binding domain. Using constructs of the transforming growth factor-beta (TGF-beta) type II receptor (RII) promoter linked to the luciferase gene, we have demonstrated that ERT activates transcription of the TGF-beta RII gene through the 5'-TTTCCTGTTTCC-3' response element spanning nucleotides +13 to +24 and multiple additional ETS binding sites between -1816 and -82 of the TGF-beta RII promoter. A specific interaction between ERT and the ETS binding sites was also demonstrated using an electrophoretic mobility shift assay. Deletion mapping of ERT protein suggests that the transactivation domain resides in the amino terminus while the DNA-binding domain is localized to the carboxyl-terminal region. Our results suggest that ERT might be a major transcription factor involved in the transcriptional regulation of the TGF-beta RII gene.
MeSH terms
Cloning, MolecularDNA, Complementary*Gene Expression RegulationHumansMolecular Sequence DataPromoter Regions, GeneticProtein BindingProtein-Serine-Threonine KinasesProto-Oncogene Proteins/*metabolismProto-Oncogene Proteins c-etsReceptors, Transforming Growth Factor beta/*geneticsTranscription Factors/*metabolismTranscription, Genetic
PMID
9417054
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Gastroenterology
AJOU Authors
함, 기백
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