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Differential expression of O6-methylguanine-DNA methyltransferase during diethylnitrosamine-induced carcinogenesis and liver regeneration in Sprague-Dawley male rats.

Lim, IK; Park, TJ; Jee, JW; Lee, MS; Paik, WK
Journal of cancer research and clinical oncology, 125(8-9):493-499, 1999
Journal Title
Journal of cancer research and clinical oncology
Differential expression of DNA-O6MeG: protein-L-cysteine S-methyltransferase (MGMT) activity and posttranslational modification of the protein during liver regeneration and carcinogenesis were compared in Sprague-Dawley male rats after partial hepatectomy and/or single i.p injection of diethylnitrosamine (DEN, 200 mg/kg). Regenerating hepatocytes after partial hepatectomy induced MGMT transiently within 3 days; however, the induction of MGMT was persistent for 2 weeks after DEN injection, and the combined treatment of DEN and partial hepatectomy maintained the elevated MGMT level for up to 4 weeks. The increased activity was transcriptionally regulated, when analyzed by Northern blot hybridization. The major active form of MGMT protein in the partially hepatectomized or DEN-treated rats was a 26-kDa or 24-kDa species respectively, which was confirmed by Western blot analysis and gel slice assay. The biological significance of the differential induction of MGMT during partial hepatectomy or DEN-induced carcinogenesis is not obvious; however, further studies on possible posttranslational modifications of MGMT protein might shed some light on the functional aspect of MGMT induction.
MeSH terms
AnimalsBlotting, NorthernBlotting, WesternDiethylnitrosamineEnzyme InductionGene Expression Regulation, Enzymologic*Gene Expression Regulation, Neoplastic*HepatectomyLiver/enzymology*Liver Neoplasms/chemically inducedLiver Neoplasms/enzymology*Liver Regeneration*MaleO(6)-Methylguanine-DNA Methyltransferase/biosynthesis*O(6)-Methylguanine-DNA Methyltransferase/geneticsO(6)-Methylguanine-DNA Methyltransferase/metabolism*RNA, Messenger/metabolismRatsRats, Sprague-DawleyTranscription, Genetic
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Journal Papers > School of Medicine / Graduate School of Medicine > Biochemistry & Molecular Biology
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