66 185

Cited 0 times in

A neutral cysteine protease of Spirometra mansoni plerocercoid invoking an IgE response.

Kong, Y; Kang, SY; Kim, SH; Chung, YB; Cho, SY
Parasitology, 114(Pt3):263-271, 1997
Journal Title
When crude extracts of Spirometra mansoni plerocercoid (sparganum) were analysed by SDS-polyacrylamide gel electrophoresis (PAGE)/immunoblot using patients' sera, IgE antibodies reacted specifically with 21, 27 and 53 kDa proteins. The 21 and 27 kDa proteins have been previously characterized as cysteine proteases. In this study, the 53 kDa protein was confirmed, by immunoprecipitation, to induce a specific IgE response. The protein was purified by affinity chromatography using an IgG1 (kappa 2) type mAb. The protein was partially sensitive to peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase F (endo F) digestion. It exhibited an endoproteinase activity in a thiol-dependent manner preferentially degrading benzoyloxycarboxyl-phenylalanyl-arginyl-4- methoxy-beta-naphthylamide (Z-phe-arg-MNA) of a panel of substrates tested. This endoprotease activity was maximal at pH 6.5 and in 0.1 M sodium phosphate. The proteolytic activity was inhibited by 10(-5) M L-trans-epoxysuccinyl-L- leucylamido-(4-guanidino)butane (E-64) and 1 mM iodoacetamide (IAA), and potentiated by dithiothreitol (DTT, 5 mM).
MeSH terms
AnimalsAntigens, Helminth/immunologyChromatography, AffinityCysteine Endopeptidases/chemistry/immunology/isolation & purification/*metabolismDithiothreitol/pharmacologyElectrophoresis, Polyacrylamide GelEnzyme Inhibitors/pharmacologyHumansHydrogen-Ion ConcentrationImmunoglobulin E/*immunologyMolecular WeightPrecipitin TestsSparganosis/immunologySparganum/*enzymology/immunologySubstrate Specificity
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Pediatrics & Adolescent Medicine
AJOU Authors
김, 성환
Files in This Item:
Full-Text Not Available.txtDownload
RIS (EndNote)
XLS (Excel)


해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.