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Grain dust induces IL-8 production from bronchial epithelial cells: the effect of dexamethasone on IL-8 production.

Authors
Park, HS; Suh, JH; Kim, HY; Kwon, OJ; Choi, DC
Citation
Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology, 82(4):371-374, 1999
Journal Title
Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology
ISSN
1081-12061534-4436
Abstract
BACKGROUND: Recent publications have suggested an active participation of neutrophils to induce bronchoconstriction after inhalation of grain dust (GD).



OBJECTIVE: To further understand the role of neutrophils in the pathogenesis of GD-induced asthma, this investigation was designed to determine whether human bronchial epithelial cells could produce IL-8 production and to observe the effect of dexamethasone on IL-8 production.



MATERIALS AND METHODS: We cultured Beas-2B, a bronchial epithelial cell line. To observe GD-induced responses, four concentrations (1 to 200 microg/mL) of GD were incubated for 24 hours and compared with those without incubation of GD. To evaluate the effect of pro-inflammatory cytokines on IL-8 production, epithelial cells were incubated with peripheral blood mononuclear cell (PBMC) culture supernatant, which was derived from the culture of PBMC from a GD-induced asthmatic subject under the exposure to 10 microg/mL of GD, and compared with those cultured without addition of PBMC supernatant. The level of released IL-8 in the supernatant was measured by enzyme-linked immunosorbent assay. To evaluate the effect of dexamethasone on IL-8 production, four concentrations (5 to 5000 ng/mL) of dexamethasone were pre-incubated for 24 hours and the same experiments were repeated.



RESULTS: There was significant production of IL-8 from bronchial epithelial cells with additions of GD in a dose-dependent manner (P < .05), which was significantly augmented with additions of PBMC supernatant (P < .05) at each concentration. Compared with the untreated sample, pretreatment of dexamethasone could induced a remarkable inhibitions (15% to 55%) of IL-8 production from bronchial epithelial cells in a dose-dependent manner.



CONCLUSION: These results suggest that IL-8 production from bronchial epithelial cells may contribute to neutrophil recruitment occurring in GD-induced airway inflammation. The downregulation of IL-8 production by dexamethasone from bronchial epithelial cells may contribute to the efficacy of this compound in reducing cellular infiltration and ultimately to its anti-inflammatory property.
MeSH terms
Bronchi/drug effects*Bronchi/immunology*Bronchi/metabolismCell-Free System/immunologyCereals/immunology*Dexamethasone/pharmacology*Dust/adverse effectsEpithelial Cells/drug effects*Epithelial Cells/immunology*Epithelial Cells/metabolismHumansInterleukin-8/biosynthesis*Interleukin-8/metabolismLeukocytes, Mononuclear/immunology
DOI
10.1016/S1081-1206(10)63286-3
PMID
10227335
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Journal Papers > School of Medicine / Graduate School of Medicine > Allergy
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박, 해심
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