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Adoptive-transfer therapy of tumors with the tumor-specific primary cytotoxic T cells induced in vitro with the B7.1-transduced MCA205 cell line.

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dc.contributor.authorKim, SJ-
dc.contributor.authorSadelain, M-
dc.contributor.authorLee, JS-
dc.contributor.authorSeong, RH-
dc.contributor.authorYun, YS-
dc.contributor.authorJang, YJ-
dc.contributor.authorChung, HY-
dc.date.accessioned2011-09-19T01:23:26Z-
dc.date.available2011-09-19T01:23:26Z-
dc.date.issued1999-
dc.identifier.issn0340-7004-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/4199-
dc.description.abstractWe show that the tumor-specific primary cytotoxic T lymphocytes (CTL) induced in vitro with the MCA205 fibrosarcoma cells transduced with the B7.1 (CD80) gene are highly effective in adoptive-transfer therapy of the parental tumors. The MCA205 fibrosarcoma cell line was transduced with the retroviral vectors encoding the B7.1 gene and tested for their efficiency as stimulators in short-term (5 days) mixed lymphocyte/tumor cell cultures with highly purified syngenic, unprimed T cells as responders. The induction of the CTL required the presence of a low dose of interleukin-2 (25 U/ml). The injection of the CTL prevented colony formation by the intravenously injected tumor cells in a lung colonization assay in which the CTL were injected after inoculation of tumor cells. We also showed that the adoptive transfer of the same T cells was effective in delaying the growth of the subcutaneously injected tumor cells. These results imply that the short-term mixed lymphocyte/tumor cell culture with the tumor cells transduced with the gene for the B7.1 costimulatory molecule is potentially a good source of CTL for adoptive-transfer therapy of tumors.-
dc.language.isoen-
dc.subject.MESHAnimals-
dc.subject.MESHAntigens, CD80-
dc.subject.MESHImmunotherapy, Adoptive-
dc.subject.MESHMice-
dc.subject.MESHMice, Inbred BALB C-
dc.subject.MESHMice, Inbred C57BL-
dc.subject.MESHSarcoma, Experimental-
dc.subject.MESHT-Lymphocytes, Cytotoxic-
dc.titleAdoptive-transfer therapy of tumors with the tumor-specific primary cytotoxic T cells induced in vitro with the B7.1-transduced MCA205 cell line.-
dc.typeArticle-
dc.identifier.pmid10022469-
dc.contributor.affiliatedAuthor장, 영주-
dc.type.localJournal Papers-
dc.citation.titleCancer immunology, immunotherapy : CII-
dc.citation.volume47-
dc.citation.number5-
dc.citation.date1999-
dc.citation.startPage257-
dc.citation.endPage264-
dc.identifier.bibliographicCitationCancer immunology, immunotherapy : CII, 47(5). : 257-264, 1999-
dc.identifier.eissn1432-0851-
dc.relation.journalidJ003407004-
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Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
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