20 220

Cited 0 times in

Glutamate neurotoxicity in mouse cortical neurons: atypical necrosis with DNA ladders and chromatin condensation.

Authors
Sohn, S; Kim, EY; Gwag, BJ
Citation
Neuroscience letters, 240(3):147-150, 1998
Journal Title
Neuroscience letters
ISSN
0304-39401872-7972
Abstract
The possibility that glutamate may induce neuronal apoptosis was examined in cultured cortical neurons. Neurons underwent widespread death 24 h following exposure to 50 microM glutamate. The glutamate neurotoxicity was blocked by inclusion of the glutamate antagonists, 10 microM MK-801 and 50 microM CNQX. The death was characterized by swelling cell body and bursting cytoplasmic membrane in the early phase of degeneration, suggesting that glutamate produces receptor-mediated excitotoxic necrosis. With blockade of excitotoxicity by addition of 10 microM MK-801 and 50 microM CNQX, cortical neurons exposed to 2 mM glutamate underwent necrosis morphologically identical to excitotoxicity but sensitive to 100 microM trolox, an antioxidant, suggesting that high doses of glutamate produce oxidative neuronal necrosis via non-receptor-mediated mechanisms. Interestingly, internucleosomal DNA fragmentation and chromatin condensation were observed in the course of glutamate-induced neuronal necrosis.
MeSH terms
AnimalsApoptosis/drug effectsCells, CulturedCerebral Cortex/*drug effects/*pathology/ultrastructureChromatin/drug effects/*metabolism/ultrastructureDNA Fragmentation/drug effectsFetusGlutamic Acid/*toxicityMiceMicroscopy, ElectronNecrosis
PMID
9502225
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
Journal Papers > School of Medicine / Graduate School of Medicine > Pharmacology
AJOU Authors
손, 성향곽, 병주
Full Text Link
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse