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Heavy chain dominance in the binding of DNA by a lupus mouse monoclonal autoantibody.

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dc.contributor.authorJang, YJ-
dc.contributor.authorLecerf, JM-
dc.contributor.authorStollar, BD-
dc.date.accessioned2011-09-19T05:03:59Z-
dc.date.available2011-09-19T05:03:59Z-
dc.date.issued1996-
dc.identifier.issn0161-5890-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/4218-
dc.description.abstractAntibodies H241 and 2C10 are lupus mouse IgG autoantibodies that bind native DNA. In previous experiments, oligonucleotide antigens affinity-labeled both H and L chains of H241 but only the H chain of antibody 2C10. Primary structures of the V regions of the 2C10 H and L chains and the H241 L chain, determined from cDNA, help to explain the previous affinity-labeling experiments. The 2C10 L chain CDRs had several Asp residues and a net negative charge of five, whereas the 2C10 H chain CDRs had four Arg residues and a net positive charge of five. The L chain CDRs of H241 had a net positive charge of one. [The H241 H chain cDNA sequence was published previously by Gangemi et al. (1993) J. Immun. 151, 4660-4671]. Plasmid vectors were used for bacterial expression of H and L chains of 2C10 alone and in combinations in single chain Fv (scFv) molecules. The H chain alone bound native DNA as well as or better than the H-plus-L chain scFv. The H chain alone also bound Z-DNA. Combination of the 2C10 H chain with the L chain of an anti-Z-DNA antibody maintained the selectivity for Z-DNA, whereas its combination with the 2C10 L chain (in the 2C10 Fab) yielded selective B-DNA binding. The results with 2C10 match other examples in which the H chain is sufficient for DNA binding but selectivity is modulated by the L chain. The H chain binding to autoantigen may reflect selective events in early stages of B cell development.-
dc.language.isoen-
dc.subject.MESHAmino Acid Sequence-
dc.subject.MESHAnimals-
dc.subject.MESHAntibodies, Antinuclear-
dc.subject.MESHAntibodies, Monoclonal-
dc.subject.MESHB-Lymphocytes-
dc.subject.MESHBase Sequence-
dc.subject.MESHCloning, Molecular-
dc.subject.MESHDNA-
dc.subject.MESHDNA Primers-
dc.subject.MESHEscherichia coli-
dc.subject.MESHImmunoglobulin Fab Fragments-
dc.subject.MESHImmunoglobulin G-
dc.subject.MESHImmunoglobulin Heavy Chains-
dc.subject.MESHImmunoglobulin Light Chains-
dc.subject.MESHImmunoglobulin Variable Region-
dc.subject.MESHLupus Erythematosus, Systemic-
dc.subject.MESHMice-
dc.subject.MESHMolecular Sequence Data-
dc.subject.MESHPolymerase Chain Reaction-
dc.titleHeavy chain dominance in the binding of DNA by a lupus mouse monoclonal autoantibody.-
dc.typeArticle-
dc.identifier.pmid8649441-
dc.identifier.urlhttp://linkinghub.elsevier.com/retrieve/pii/0161589095000941-
dc.contributor.affiliatedAuthor장, 영주-
dc.type.localJournal Papers-
dc.citation.titleMolecular immunology-
dc.citation.volume33-
dc.citation.number2-
dc.citation.date1996-
dc.citation.startPage197-
dc.citation.endPage210-
dc.identifier.bibliographicCitationMolecular immunology, 33(2). : 197-210, 1996-
dc.identifier.eissn1872-9142-
dc.relation.journalidJ001615890-
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Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
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