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Cytokine-induced production of macrophage inflammatory protein-1alpha (MIP-1alpha) in cultured human astrocytes.

Authors
Miyamoto, Y; Kim, SU
Citation
Journal of neuroscience research, 55(2):245-251, 1999
Journal Title
Journal of neuroscience research
ISSN
0360-40121097-4547
Abstract
Macrophage inflammatory protein-1alpha (MIP-1alpha) is a member of a superfamily of inflammatory cytokines termed chemokines, and it has been implicated in the pathogenesis of several human diseases with inflammatory components. It has been known that MIP-1alpha plays a role in recruiting and activating mononuclear phagocytes in the central nervous system (CNS), and that astrocytes and microglia are sources of this chemokine. However, details of the regulation of MIP-1alpha production by these glial cells are not known. In the present study, expression of MIP-1alpha was determined in purified cultures of human astrocyte. MIP-1alpha mRNA levels in human astrocyte cell preparations were determined by reverse transcription polymerase chain reaction (RT-PCR) and amount of MIP-1alpha protein secreted into culture supernatants by human astrocytes was assayed by enzyme-linked immunosorbent assay (ELISA). Under the unstimulated conditions, human astrocytes did not express MIP-1alpha message or protein, indicating that human astrocytes do not constitutively carry MIP-1alpha message. Following treatment with interleukin-1beta (IL-1beta), human astrocytes demonstrated increased message and protein expression for MIP-1alpha, while other immune modulators such as interferon-gamma (IFN)-gamma, tumor necrosis factor-alpha (TNF-alpha), granulocyte-macrophage colony-stimulating factor (GM-CSF), lipopolysaccharide, or phorbol ester (a protein kinase C activator) did not induce MIP-1alpha expression in human astrocytes.
MeSH terms
Astrocytes/drug effectsAstrocytes/metabolism*Cells, CulturedChemokine CCL3Chemokine CCL4Cytokines/pharmacology*Enzyme-Linked Immunosorbent AssayHumansMacrophage Inflammatory Proteins/biosynthesis*RNA, Messenger/biosynthesisRNA, Messenger/geneticsReverse Transcriptase Polymerase Chain ReactionStimulation, Chemical
DOI
10.1002/(SICI)1097-4547(19990115)55:2<245::AID-JNR12>3.0.CO;2-Q
PMID
9972827
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Neurology
AJOU Authors
김, 승업
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