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Process extension and intracellular Ca2+ in cultured murine oligodendrocytes

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dc.contributor.authorYoo, AS-
dc.contributor.authorKrieger, C-
dc.contributor.authorKim, SU-
dc.date.accessioned2011-09-21T01:53:06Z-
dc.date.available2011-09-21T01:53:06Z-
dc.date.issued1999-
dc.identifier.issn0006-8993-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/4237-
dc.description.abstractPrevious investigations have shown that phorbol esters stimulate process extension in oligodendrocytes (OL), likely by the activation of protein kinase C (PKC). In this report, we demonstrate that treatment of OL with 4beta-phorbol-12, 13-dibutyrate (PDB; 0.1-1 microM) resulted in an increase in intracellular Ca2+ concentration ([Ca2+]i) from 94+/-2 nM (mean+/-S.E.M.) to 244+/-10 nM. This increase was produced by Ca2+ influx through a La3+-insensitive pathway. Changes in [Ca2+]i were also produced by modifying the extracellular Ca2+ concentration ([Ca2+]o) where [Ca2+]i was increased by elevations in [Ca2+]o. In parallel experiments we found that increased [Ca2+]o alone, without concurrent phorbol ester application, resulted in increased OL process extension as determined by the percent of OL with long processes (greater than 3 times the cell body diameter). These results demonstrate that increasing [Ca2+]o stimulates OL process outgrowth. Furthermore, both elevations in [Ca2+]o and PDB exposure increase [Ca2+]i, suggesting that some of the effects of phorbol esters on OL process extension are likely mediated by changes in [Ca2+]i.-
dc.language.isoen-
dc.subject.MESHAnimals-
dc.subject.MESHBiological Transport-
dc.subject.MESHCalcium-
dc.subject.MESHCalcium Channels-
dc.subject.MESHCarcinogens-
dc.subject.MESHCell Division-
dc.subject.MESHCells, Cultured-
dc.subject.MESHChelating Agents-
dc.subject.MESHEgtazic Acid-
dc.subject.MESHEnzyme Inhibitors-
dc.subject.MESHFluorescent Dyes-
dc.subject.MESHFura-2-
dc.subject.MESHIndoles-
dc.subject.MESHLanthanum-
dc.subject.MESHMice-
dc.subject.MESHMice, Inbred BALB C-
dc.subject.MESHOligodendroglia-
dc.subject.MESHPhorbol 12,13-Dibutyrate-
dc.subject.MESHProtein Kinase C-
dc.titleProcess extension and intracellular Ca2+ in cultured murine oligodendrocytes-
dc.typeArticle-
dc.identifier.pmid10320689-
dc.identifier.urlhttp://linkinghub.elsevier.com/retrieve/pii/S0006-8993(99)01282-2-
dc.contributor.affiliatedAuthor김, 승업-
dc.type.localJournal Papers-
dc.citation.titleBrain research-
dc.citation.volume827-
dc.citation.number1-2-
dc.citation.date1999-
dc.citation.startPage19-
dc.citation.endPage27-
dc.identifier.bibliographicCitationBrain research, 827(1-2). : 19-27, 1999-
dc.identifier.eissn1872-6240-
dc.relation.journalidJ000068993-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Neurology
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