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Presenilin 1 mediates protein kinase C dependent alpha-secretase derived amyloid precursor protein secretion and mitogen-activated protein kinase activation in presenilin 1 transfected human embryonic kidney 293 cell.

Shin, JE; Koh, JY; Mook-Jung, I
Neuroscience letters, 269(2):99-102, 1999
Journal Title
Neuroscience letters
We investigated the role of presenilin 1 (PS1) in the secretion of alpha-secretase derived amyloid precursor protein (sAPP alpha) and associated intracellular signaling pathways. Human embryonic kidney (HEK) 293 cells were transfected with exon 9 deletion (deltaE9) mutant PS1 cDNA in an ecdyson-inducible system. sAPPalpha secretion was lower in the mutant PS1 expressing cells compared with non-expressing cells. When activated by PDBu, secretion of sAPPalpha and the level of phosphorylated mitogen activated protein kinase (MAPK) were greatly enhanced in deltaE9 PS1 uninduced cells, but not in the mutant PS1 induced cells. PD98059, a MAPK inhibitor, blocked PDBu induced sAPPalpha secretion from deltaE9 PS1 uninduced cells but had no effect on the mutant PS1 induced cells. These data indicate that PS1 mediates PDBu-induced sAPPalpha secretion and MAPK activation.
MeSH terms
Alzheimer Disease/enzymologyAlzheimer Disease/physiopathology*Amyloid Precursor Protein SecretasesAmyloid beta-Protein Precursor/secretion*Aspartic Acid EndopeptidasesCalcium-Calmodulin-Dependent Protein Kinases/metabolism*Cell LineEndopeptidases/physiology*Enzyme ActivationHumansMembrane Proteins/geneticsMembrane Proteins/physiology*MutationPresenilin-1Protein Kinase C/physiology*
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Journal Papers > Research Organization > Brain Disease Research Center
AJOU Authors
묵, 인희
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