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Engraftable human neural stem cells respond to developmental cues, replace neurons, and express foreign genes.

Authors
Flax, JD; Aurora, S; Yang, C; Simonin, C; Wills, AM; Billinghurst, LL; Jendoubi, M; Sidman, RL; Wolfe, JH; Kim, SU; Snyder, EY
Citation
Nature biotechnology, 16(11):1033-1039, 1998
Journal Title
Nature biotechnology
ISSN
1087-01561546-1696
Abstract
Stable clones of neural stem cells (NSCs) have been isolated from the human fetal telencephalon. These self-renewing clones give rise to all fundamental neural lineages in vitro. Following transplantation into germinal zones of the newborn mouse brain they participate in aspects of normal development, including migration along established migratory pathways to disseminated central nervous system regions, differentiation into multiple developmentally and regionally appropriate cell types, and nondisruptive interspersion with host progenitors and their progeny. These human NSCs can be genetically engineered and are capable of expressing foreign transgenes in vivo. Supporting their gene therapy potential, secretory products from NSCs can correct a prototypical genetic metabolic defect in neurons and glia in vitro. The human NSCs can also replace specific deficient neuronal populations. Cryopreservable human NSCs may be propagated by both epigenetic and genetic means that are comparably safe and effective. By analogy to rodent NSCs, these observations may allow the development of NSC transplantation for a range of disorders.
MeSH terms
AnimalsAnimals, NewbornBiotechnologyBrain/cytology/growth & development/surgery*Brain Tissue TransplantationCell MovementCells, Cultured*Fetal Tissue TransplantationGene TherapyGenetic EngineeringHumansMiceNeurons/cytology/physiology/*transplantation*Stem Cell TransplantationStem Cells/cytology/physiologyTay-Sachs Disease/enzymology/genetics/therapyTransplantation, Heterologousbeta-N-Acetylhexosaminidases/deficiency/genetics
DOI
10.1038/3473
PMID
9831031
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Neurology
AJOU Authors
김, 승업
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