Aflatoxin Related p53 Codon 249 Point Mutation in Korean Hepatocellular
한국의 간세포암과 Aflatoxin 관련 P53 codon249 점돌연변이와의 관계
왕, 희정; 홍, 성우; 박, 영석; 김, 욱환; 김, 명욱
Korean journal of hepato-biliary-pancreatic surgery, 2(1):79-83, 1998
Korean journal of hepato-biliary-pancreatic surgery
Purpose: P53 point mutation, especially G to T transversion at codon 249, has been found in many cases of the aflatoxin-related hepatocellular carcinoma(HCC). This study was aimed to exam the mutations of the third base at codon 249 of the p53 gene in Korean HCC.
Materials and Methods:Forty two patients with resectable HCC were enrolled. Genomic DNA was prepared by proteinase K digestion and phenol/chloroform extractions. A part of DNA including axon 7 of p53 was amplified by PCR. Restriction fragment polymorphism (RFLP) was analyzed after treatment of Hae III restriction enzyme. And the point mutation were confirmed using the direct sequencing method.
Results: P53 point mutation, G to T transversion at codon 249 was observed in only one tissue specimen(2.4%).
Conclusion: P53 point mutation at codon 249 does not correlate with HCC in Korea.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
Total Visit :2,768,004
Total Download :1,129,698
Today View :1,393
Ajou University Medical Information & Media Center 164 Worldcup-ro Yeongtong-gu Suwon 16499 Korea / TEL : 031-219-5312 / FAX : 031-219-5314 Copyright (c) Ajou University Medical Information & Media Center All Rights Reserved. AJOU Open Repository는 국립중앙도서관 OAK 보급사업으로 구축되었습니다.