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Effect of Hepatocyte Growth Factor on the Expression of E-cadherin in Gastric Carcinoma Cell Lines

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dc.contributor.author한, 상욱-
dc.contributor.author이, 원흥-
dc.contributor.author김, 욱환-
dc.contributor.author김, 명욱-
dc.contributor.author이, 재호-
dc.contributor.author송, 상용-
dc.contributor.author이, 건욱-
dc.date.accessioned2011-11-24-
dc.date.available2011-11-24-
dc.date.issued2000-
dc.identifier.issn0496-6872-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/4526-
dc.description.abstractPURPOSE: Previously, we reported that the expression of E-cadherin was significantly decreased according to the increase of the level of hepatocyte growth factor (HGF) in gastric cancer tissue. In this work, the effect of HGF on the cell-cell adhesion and intracellular distribution of E-cadherin in the gastric carcinoma cell lines were studied.
MATERIALS AND METHODS: Western blot analysis was performed to confirm the presence or abscence of c-Met and E-cadherin in SNU-1, 5, and 16 cells. Tyrosine phosphorylation of c-Met, E-cadherin, alpha-, beta-, gamma-catenins was checked by immunoprecipitation. The morphologic changes induced by HGF were studied with immunocytochemical staining. Functional proportion of E-cadherin was estimated by cell fractionation. The effect of HGF on cell proliferation and invasion was also assessed.
RESULTS: Among SNU-1, 5, and 16 cell lines, only SNU-16 cells expressed both E-cadherin and c-Met. A morphological change from epithelial shape to fibroblastic one was observed in the SNU-16 cells after treatment with HGF. In addition, E-cadherin expression of the SNU-16 cells was shifted from the membrane and to the cytoplasm, and the functional fraction of E-cadherin was decreased in the SNU-16 cells treated with HGF. On the other hand, HGF increased the proliferation and invasion of the SNU-16 cells.
CONCLUSION: These results suggest that HGF may regulate cell adhesion in gastric carcinomas via the cellular redistribution and functional change of E-cadherin.
en
dc.formatapplication/pdf-
dc.language.isoko-
dc.titleEffect of Hepatocyte Growth Factor on the Expression of E-cadherin in Gastric Carcinoma Cell Lines-
dc.title.alternative위암세포주에서 간세포성장인자가 E-cadherin의 발현에 미치는 영향-
dc.typeArticle-
dc.identifier.urlhttps://www.e-crt.org/journal/view.php?number=724-
dc.subject.keywordStomach neoplasm-
dc.subject.keywordHepatocyte growth factor-
dc.subject.keywordc-Met gene-
dc.subject.keywordE-cadherin-
dc.subject.keywordInvasion-
dc.contributor.affiliatedAuthor한, 상욱-
dc.contributor.affiliatedAuthor김, 욱환-
dc.contributor.affiliatedAuthor김, 명욱-
dc.contributor.affiliatedAuthor이, 재호-
dc.type.localJournal Papers-
dc.citation.titleJournal of Korean Cancer Association-
dc.citation.volume32-
dc.citation.number5-
dc.citation.date2000-
dc.citation.startPage852-
dc.citation.endPage862-
dc.identifier.bibliographicCitationJournal of Korean Cancer Association, 32(5). : 852-862, 2000-
dc.relation.journalidJ004966872-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Surgery
Journal Papers > School of Medicine / Graduate School of Medicine > Biochemistry & Molecular Biology
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