Production of Monoclonal Anti-idiotypic Antibody to Monoclonal Anti-DNA Antibody
Anti-DNA 단클론 항체에 대한 Anti-idiotype 단클론 항체의 생산
권, 명희; 강, 재승; 신, 호준; 장, 영주; 박, 선; 이, 미리나; 김, 형일
Taehan Myŏnyŏk Hakhoe chi, 20(2):109-117, 1998
Taehan Myŏnyŏk Hakhoe chi; The Korean journal of immunology : official journal of the Korean Society for Immunology; 대한면역학회지
It has been thought that autoimmune diseases like systemic lupus erythematosus and
rhumatoid athritis are closely associated with anti-DNA antibodies (Abs). In studies of the control for anti-DNA Ab generation, an understanding of the regulatory mechanisms by anti-idiotypic Abs that influence the production of anti-DNA Abs would be facillitated by the availability of the hybridomas producing the pairs of DNA-specific and anti-DNA's idiotope-specific monoclonal antibodies (mAbs). We have produced a series of anti-DNA mAbs and then monoclonal anti-idiotypic Ab directed against idiotypic determinant of the 3D8 mAb that has the highest affinity to dsDNA and
SSDNA among the anti-DNA mABs that we had obtained. The spleen cells of the MRL-lpr/lpr, autoimmune prone, mice were fused with P3×63Ag8.653 myeloma cells to obtain anti-DNA Ab secreting hybridomas. Out of the fourteen clones that showed strong binding to ssDNA, four clones had cross-reactivity with dsDNA whereas none of these clones reacted with left-handed z-DNA. The binding activities of the anti-DNA mAbs to various synthetic polynucleotide sequences were different respectively.
Anti-idiotypic mAbs were generated by the fusion of myeloma cells and spleen cells
from the Balb/c mice immunized with 3D8-Fab. We have produced two anti-idiotypic
mAbs, B7 (IgG2a/ ) and 02F3 (IgM/ ), which were specific to 3D8-Fab and cloned the
variable region of the heavy chain from the O2F3 hybridoma.
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