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E1B-19k does not Localize in Mitochondria nor Dimerize Bax even with the Staurosporine

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dc.contributor.author윤, 수한-
dc.contributor.author김, 진영-
dc.contributor.author박, 승우-
dc.contributor.author안, 영환-
dc.contributor.author안, 영민-
dc.contributor.author조, 기홍-
dc.contributor.author조, 경기-
dc.date.accessioned2011-12-13-
dc.date.available2011-12-13-
dc.date.issued2000-
dc.identifier.issn1225-8245-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/4823-
dc.description.abstractPurpose:The subcellular localization of E1B-19k has been known cytosol or nuclear membrane by immuno-histochemical staining and could dimerize with Bax to regulate cell death also known by the in-vitro immunoprecipitation. We planed to confirm this dimerization of E1B-19k with Bax in vivo in Cos-7 cells by using green fluorescent protein.



Material and Method:We cloned E1B-19k and Bax into C3-EGFP. C3-EGFP-E1B-19k, C3-EGFP-Bax, and C3-EGFP-E1B-19k and pcDNA3-Bax were transfected into Cos-7 cells. We explored location of E1B-19k and Bax, and confirmed its dimerization with Bax in transfected living healthy Cos-7 cells by following green fluorescent protein of E1B-19k on the confocal microscope.



Results:E1B-19k was located diffusely in cytoplasm and in nucleus but not in mitochondria. It prevented cell death from the apoptosis by staurosporine but its location was not changed. GFP-E1B-19k is not changed its intracellular location with Bax even with staurosporine.



Conclusion:These results support that E1B-19k does not localize in mitochondria nor dimerize with Bax even with staurosporine. We could anticipate E1B-19k prevent cell death via the other dimerizing partner or pathways.
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dc.language.isoko-
dc.titleE1B-19k does not Localize in Mitochondria nor Dimerize Bax even with the Staurosporine-
dc.title.alternativeE1B-19k의 세포내 위치와 Bax와의 Dimerization에 관한 연구-
dc.typeArticle-
dc.identifier.urlhttp://www.jkns.or.kr/htm/abstract.asp?no=0042000102&m=0-
dc.subject.keywordE1B-19k-
dc.subject.keywordBax-
dc.subject.keywordDimerization-
dc.subject.keywordApoptosis-
dc.subject.keywordStaurosporine-
dc.subject.keywordGreen fluorescent protein-
dc.type.localJournal Papers-
dc.citation.titleTaehan Sinkyŏng Oekwa Hakhoe chi-
dc.citation.titleJournal of Korean Neurosurgical Society-
dc.citation.title대한신경외과학회지-
dc.citation.volume29-
dc.citation.number6-
dc.citation.date2000-
dc.citation.startPage725-
dc.citation.endPage730-
dc.identifier.bibliographicCitationTaehan Sinkyŏng Oekwa Hakhoe chi, 29(6):725-730, 2000-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Neurosurgery
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