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The Effect of Retinoids in Medulloblastoma Cell Culture

Other Title
수아세포종 세포 배양에서 레티노이드의 세포증식억제 효과
Authors
윤, 수한; 김, 진영; 박, 승우; 안, 영환; 안, 영민; 조, 기홍; 조, 경기
Citation
Taehan Sinkyŏng Oekwa Hakhoe chi, 28(12):1773-1781, 1999
Journal Title
Taehan Sinkyŏng Oekwa Hakhoe chi; Journal of Korean Neurosurgical Society; 대한신경외과학회지
ISSN
1225-8245
Abstract
The retinoic acid, one of the most popular agents for chemoprevention can inhibit the proliferation of many cancer cells including neuroblastoma and glioblastoma but there is increasing demand reaccessing its in vitro inhibitory effect on the tumor proliferation because of poor responsiveness from recent clinical trial for malignant brain tumor with retinoic acid. If was known to effect on tumor cells by diffferentiation and apoptosis so that its effect was expected greater in pediatric brain tumor than in adult brain tumor, but there is no report on the effect of retinoic acid in medulloblastoma cell proliferation except all-trans and 13-cis retinoic acid. Therefore, we compared the effects of all-trans, 13-cis, and 9-cis retinoic acid and N-4-hydroxyphenyl)retinamide to inhibit proliferation of medulloblastoma tumor cells. Medulloblastoma cells were derived from primary culture of patient's specimen. We estimated the rate of growth inhibition of each tumor cells using MTT assay in the concentration from 10-12M to 10-5M of all-trans, 13-cis, and 9-cis retinoic acid and N-4-hydroxyphenyl)retinamide. Medulloblastoma cells showed more than 30% growth inhibition by all-trans, 12% by 13-cis, 20% by 9-cis retinoic acid and 7% by N-4-hydroxyphenyl)retinamide at 14 days culture on the concentration of 10-6M. In conclusion, significanty and dramatic effect by, especially, all-trans retinoic acid, moderate response by 13-cis retinoic acid and variable or poor response by 9-cis retinoic acid and N-4-hydroxyphenyl) retinamide.
Keywords
All-trans retinoic acid13-cis retinoic acid9-cis retinoic acidN-4-hydroxyphenylretinamideMTT assayChemoprevention
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Journal Papers > School of Medicine / Graduate School of Medicine > Neurosurgery
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