In Vitro Blood-brain Barrier Model of Astrocyte and Endothelial Monolayer Co-culture Using Collagen-coated Microporous Membrane

Abstract

There are three types of the in vitro blood-brain barrier models that are endothelial monolayer, astrocyte and endothelial monolayer co-culture, and benign glioma and endothelial monolayer co-culture. We made these three types of models and evaluated permeability ratio for four molecular weight(MW 373, 4400, 9300, 38900) and electrical resistance of endothelial monolayer in each type. Astrocyte and endothelial monolayer co-culture showed the lowest permeability ratio and highest electrical resistance among them and that suggested astrocyte and endothelial monolayer co-culture was best for the in-vitro blood-brain barrier model.