Taehan Haebu Hakhoe chi; The Korean journal of anatomy; 대한해부학회지
P19, murine embryonal carcinoma(EC) cells can be induced to differentiate into neurons in the presence of retinoic acid(RA). To investigate neuronal differentiation of P19 cells in details, P19 aggregates were obtained in the presence or absence of RA,
ascorbic acid(AA) and 2-mercaptoethanol(2-ME) in bacteriological Petri dishes. When the aggregates were transferred intko the serum depleted medium, P19 cells exhibited dramatic morphological changes. Cells contained long and thin processes as detected in differentiated neurons. Western blot analysis showed that treatment of RA and AA induced expression of neuron-specific markers such as NCAM, NSE and Tuj1. Expression of GFAP was not detected, suggesting that P19 cells differentiate into
neurons under our experimental condition. Immunocytochemical studies also revealed that treatment of RA and AA increased expression of NCAM and Tuj1. On the ontrary, 2-ME was ineffective in the neuronal differentiation of P19 cells, which is consistent the results from the western blot analysis. These results suggest that differentiated P19 cells have similar characteristics to those of typically differentiated neurons. This study also suggests that P19 cells may provide useful tools to study neuronal differentiation in vitro.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
Total Visit :4,501,473
Total Download :1,944,879
Today View :1,617
Ajou University Medical Information & Media Center 164 Worldcup-ro Yeongtong-gu Suwon 16499 Korea / TEL : 031-219-5312 / FAX : 031-219-5314 Copyright (c) Ajou University Medical Information & Media Center All Rights Reserved. AJOU Open Repository는 국립중앙도서관 OAK 보급사업으로 구축되었습니다.