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The Efficient Gene Delivery into Human Mesenchymal Stem Cells Using Retroviral Vectors

Authors
Kim, SS; Kim, BJ; Suh-Kim, H
Citation
Taehan Haebu Hakhoe chi, 36(5):381-387, 2003
Journal Title
Taehan Haebu Hakhoe chi; The Korean journal of anatomy; 대한해부학회지
ISSN
1225-1305
Abstract
Human mesenchymal stem cells (hMSCs) are multipotent stem cells that can differentiate into several mesenchymal lineage cells. In this study, we established an efficient method for gene delivery into these cells. Non-viral transfection reagents that were commercially available yielded 5% efficiency. In contrast, a retroviral vector yielded more than 46% transduction, which was further increased to 90% by repetitive infection. Retroviral transduction did not alter the multipotency of hMSCs. Thus, the cells retained the potential to differentiate into adipogenic, chondrogenic, or osteogenic lineages.

The conditions established in this study will contribute to development of trans-differentiation methods of hMSCs into non-mesodermal lineage cells and thereby facilitate their possible use as vehicles for autologous transplantation in both cell and gene therapy for various diseases.
Keywords
Mesenchymal stem cellsGene therpayRetrovirusTransfection
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Anatomy
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