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The Effects of Intrasplenic Transplantation of Hepatocytes on Rats with Acute Liver Failure Induced by a 90% Hepatectomy

Other Title
90% 간절제술로 유도된 백서의 전격성 간부전모델에서 비장내 간세포 이식의 효과에 관한 연구
Authors
김, 욱환; 이, 재호; 한, 상욱; 진, 윤미; 곽, 연식; 왕, 희정; 김, 명욱
Citation
Taehan Oekwa Hakhoe, 56(1):27-34, 1999
Journal Title
Taehan Oekwa Hakhoe; 대한외과학회지
ISSN
1226-0053
Abstract
Background : Acute liver failure either after liver resection or as part of underlying

liver disease is still associated with high mortality. Various treatments have been tried

to improve liver function during acute liver failure, including metabolic and nutritional

support, hemodialysis, hemoperfusion, plasmapheresis, and hepatocyte and liver

transplantation. Hepatocyte transplantation in various forms has attracted attention

recently. We investigated whether allogeneic isolated hepatocytes transplanted in the

spleen would prolong survival, facilitate liver regeneration, and improve biochemical

parameters in rats with acute lived failure induced by a 90% hepatectomy.

Methods : Allogeneic male Sprague-Dawley rats were used. Group Ⅰ rats (n=26)

received an intrasplenic injection of 2×107 hepatocytes in 0.3 ㎖ of

Dulbecco's modified Eagle's medium (DMEM), and 1 days later acute liver failure was

induced. Group Ⅱ acute-liver-failure rats (n=24) received an intrasplenic injection of

DMEM. The survival time was determined for 22 rats in Group I and for 20 rats in

Group Ⅱ. The remaining 8 (4/each group) acute liver failure rats were used to assess

the liver function and regeneration.

Results : The survival was longer and the number of long-term survivors was

greater for Group Ⅰ rats than for the Group Ⅱ controls. At 24 hour after the

hepatectomy, Group Ⅰ rats had lower ammonia, lower total bilirubin, lower activities of

liver enzymes, and higher glucose levels than did Group Ⅱ rats. In Group Ⅰ, there was

significant increase in the ratio of the weight of the remnant liver lobes to the body

weight.

Conclusion : Compared with the Control group intrasplenic hepatocyte transplantation

in acute liver failure rats acts as a bridge to support experimental rats in going from

acute or fulminant liver failure to liver regeneration or compensation, prolongs survival

in rats with acute liver failure induced by a 90% hepatectomy, and improves the

biochemical parameters, except for the albumin levels and prothrombin time.

Transplantation of hepatocytes may be beneficial in supporting a liver which has been

acutely devastated by a 90% hepatectomy.
Keywords
Hepatocyte transplantationAcute liver failure90% hepatectomy
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Surgery
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