40 150

Cited 0 times in

UTP and ATPgammaS Induce Mucin Secretion via Ca2+ Dependant Pathways in Human Nasal Epithelial Cells

Other Title
정상 코점막 상피세포에서 UTP와 ASPγS에 의한 Ca2+ 의존성 경로를 통한 점액 분비
Authors
김, 현준; 최, 재영; 김, 진영; 서, 용석; 이, 정권; 윤, 주헌
Citation
Taehan Ibi Inhukwa Hakhoe chi, 46(4):302-308, 2003
Journal Title
Taehan Ibi Inhukwa Hakhoe chi; Journal of the Korean Otolaryngological Society; 대한이비인후과학회지
ISSN
1225-035X
Abstract
Backgroud and Objectives: Nucleotides such as adenosine triphosphate (ATP) and uridine-5-triphosphate (UTP) play fundamental roles in the early stage of secretion in nasal epithelial cells via P2Y receptor. In the present study, we examined the expression pattern of P2Y subtypes and their functions on Ca2+ influx ([Ca2+]i) in normal human nasal epithelial (NHNE) cells. We also examined the effect of UTP (agonist for P2Y2) and ATPδS (agonist for P2Y11) on mucin secretion and mucin gene expression.



Materials and Method: The expression pattern of P2Y receptors and mRNA levels of MUC5AC, MUC5B and MUC8 were examined after treatment with UTP and ATPδS by RT-PCR. Mucin was quantitated by immunoblotting assay. We measured the [Ca2+]i in NHNE cells with a double perfusion chamber.



Results: Two uracil-sensitive receptors (P2Y2, P2Y4) and two adenine-sensitive receptors (P2Y1, P2Y11) were expressed in NHNE cells. UTP and ATPδS increased [Ca2+]i via caffeine-sensitive pathways, and these two agonists stimulated mucin secretion to a similar magnitude without their gene enhancement. In addition, the mucin stimulatory effects subsided when the intracellular Ca2+ was removed by 2-bis (2-aminophenoxy) ethane-N,N,N’,N’-tetraacetic acid-acetoxymethyl ester.



Conclusion: Our study showed that P2Y2 and P2Y11 receptors were expressed in NHNE cells and that their agonists, UTP and ATPδS, act as secretogogues on mucin secretion via Ca2+-dependent pathways.
Keywords
NucleotidesNasal MucosaReceptors purinergicCaffeine
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Otolaryngology
Files in This Item:
Full-Text Not Available.txtDownload
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse