Cited 0 times in Scipus Cited Count

Effect of HGF in Proliferation, Dispersion and Migration of Hypopharyngeal Squamous Cell Carcinoma

Other Title
간세포성장인자가 하인두 편평세포암 세포주의 증식, 분산과 이동에 미치는 영향
Authors
김, 철호  | 문, 성균  | 배, 준호  | 이, 재호  | 채, 은창
Citation
Journal of the Korean Otolaryngological Society, 48(2). : 208-215, 2005
Journal Title
Journal of the Korean Otolaryngological Society
ISSN
1225-035X
Abstract
BACKGROUND AND OBJECTIVES: Hepatocyte growth factor (HGF) is known to stimulate motility, invasiveness, proliferation, and morphogenesis of endothelial cells. Recent reports revealed that this growth factor is also related to tumor invasion and metastasis. We examined the role of HGF/c-Met on the proliferation, dispersion and migration of FaDu cell, a hypopharyngeal squamous cell carcinoma cell line.
MATERIALS AND METHOD: We performed RT-PCR and Western blot in FaDu. Proliferation of the FaDu cells was assayed by counting the number of cells after treatment by HGF of different concentrations of 0, 10, 30 ng/mL. Dispersion of the cells was observed by measuring the separation and morphologic changes of cells after the colony of FaDu cells was formed in the media and then treated with HGF of 10 ng/mL or 30 ng/mL for 24 hours. Tumor cell migration was assessed by wound healing assay. Lastly, we examined the enhancement of HGF production in human fibroblast (MRC-5) by putative inducer secreted from FaDu cells.
RESULTS: The expresssion of c-Met mRNA and protein was detected in the hypopharyngeal cell line while that of HGF was not. Exogenous HGF significantly enhanced the growth of FaDu in a dose-dependent manner 30 ng/ml (p<0.05). HGF stimulated the dispersion and enhanced the migration of cancer cells in a dose-dependent manner (p<0.05). HGF produced by human stromal fibroblast (MRC-5) was increased by a certain inducer originated from FaDu cells (p<0.05).
CONCLUSION: These results suggest that HGF may play an important role in the progression of hypopharyngeal cancer through the enhancement of proliferation, dispersion and migration.
Keywords

Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Otolaryngology
Ajou Authors
김, 철호  |  문, 성균  |  배, 준호  |  이, 재호
Files in This Item:
Journal of the Korean Otolaryngological Society_48(2)_208-215.pdfDownload
Export

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse