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Effect of HGF in Invasion of Hypopharyngeal Squamous Cell Carcinoma Cell Line

Other Title
간세포성장인자가 하인두 편평세포암 세포주에서 Matrix Metalloproteinase(MMP)-2, 9과 Urokinase-Type
Authors
김, 철호; 문, 성균; 배, 준호; 이, 재호; 최, 은창
Citation
Taehan Ibi Inhukwa Hakhoe chi, 48(6):788-795, 2005
Journal Title
Taehan Ibi Inhukwa Hakhoe chi; Journal of the Korean Otolaryngological Society; 대한이비인후과학회지
ISSN
1225-035X
Abstract
Background and Objectives: Recent reports revealed that hepatocyte growth factor (HGF) is related to tumor invasion and metastasis. Activation of the urokinase-type plasminogen activator (u-PA)/plasmin proteolytic network and matrix metalloproteinase has been shown to play a key role in tumor invasion and dissemination of various malignancies. So we examined the effect of HGF/c-Met on MMP-2, 9 and u-PA in FaDu cell, a hypopharyngeal squamous cell carcinoma cell line.



Materials and Method: We performed RT-PCR and Western blot in FaDu. Tumor cell invasiveness was assessed by the membrane invasion assay (using Transwell chamber). To examine the role of MMP-2, 9 and the relation between HGF and MMP in the invasion of hypopharyngeal cancer, RT-PCR and zymography were performed in FaDu cells. We tested to confirm the HGF-mediated plasmin activation.



Results: The expressions of c-Met mRNA and protein were detected in the hypopharyngeal cell line while that of HGF was not. HGF markedly enhanced the invasion of cancer cells in a Transwell invasion chamber in a dose-dependent manner (p<0.05). The expression of MMP was detected in hypopharyngeal cancer cells and exogenous HGF slightly enhanced the induction of MMP-2 activity in zymogram analysis. The activity of u-PA was detected in FaDu and HGF (above 10 units/mL) enhanced the activity of u-PA (p<0.05).



Conclusion: These results suggest that HGF may play an important role in hypopharyngeal cancer through the activation of u-PA and matrix metalloproteinase.
Keywords
Hepatocyte growth factorc-MetHypopharyngeal cancerInvasionUrokinase-typeplasminogen activatorMatrix metalloptoteinase
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Journal Papers > School of Medicine / Graduate School of Medicine > Otolaryngology
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