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Apoptosis of human luteinized granulosa cell via change of mitochondria

Other Title
ATP 에 의해 유도된 인간 난소 황체화 과립세포의 세포자멸사에 관한 연구
Authors
김, 미란; 박, 동욱; 김, 영아; 조, 태섭; 황, 경주; 임, 윤경; 민, 철기
Citation
Taehan Sanbuinkwa Hakhoe chapchi, 44(11):2091-2097, 2001
Journal Title
Taehan Sanbuinkwa Hakhoe chapchi; Korean journal of obstetrics and gynecology; 대한산부인과학회잡지
ISSN
0494-4755
Abstract
Objective : To determine the effects of extracellular ATP on mitochondrial function and apoptosis during human luteinized granulosa cell cultures. Methods : The addition of various concentrations of ATP (0, 0.1, 0.25, 0.5, 0.75 mM) to luteinized granulosa cells obtained during In vitro fertilization ovum pickup procedures. After culture for 24 hours, purinoceptor activity and functional changes in mitochondria were measured by patch clamp, flow cytometry, and confocal microscopy. Results : Measurement by patch clamp of the granulosa cell membrane potential after ATP addition to cultured granulosa cells showed that both the inward and outward currents were expressed. After treatment of the granulosa cells with JC-1, measurement of the mitochondrial activity by confocal microscopy showed that the with increasing concentrations of ATP the relative ratio of undamaged mitochondria (red/green ratio) tended to decrease (P=0.027). After double staining of the cultured granulosa cells with Annexin V and Propidium Iodide, quantitative flow cytometry analysis showed that apoptosis increased with increasing concentrations of ATP (7.88%, 8.44%, 11.40%, 13.52%, 18.57%). Conclusion : The results of this study shows that apoptosis of granulosa cells increases with increasing extracellular ATP concentrations in cultured human luteinized granulosa cells. This is observed to be a consequence of cell membrane purinoceptor activity and functional changes in the mitochondria. It is therefore thought that remodelling processes of the ovarian tissue is regulated by neuroendocrine factors of the extracellular ATP.
Keywords
PurinoceptorApoptosisLuteinized granulosa cell
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Obstetrics & Gynecology
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